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中北 愼一 ナカキタ シンイチ

所属
医学部
医学科
総合生命科学研究センター
職名准教授
Last Updated :2021/11/22

研究者基本情報

学歴

  • 1994年04月 - 1998年09月, 大阪大学, 大学院理学研究科

学位

  • 理学, 大阪大学

所属学協会

  • 日本糖質学会

経歴

  •  - 現在, 香川大学

研究活動情報

研究分野

  • ライフサイエンス / 機能生物化学

研究キーワード

    希少糖, ウイルス, 構造解析, 糖鎖

論文

  • Preparation of a molecular library of branched β-glucan oligosaccharides derived from laminarin, Journal of Applied Glycoscience, 65, (4) 45 - 49,   2018年, [査読有り]
  • 糖質関連分子とバイオセンサ, 宮西 伸光, 中北愼一, 住吉渉, 平林淳, 応用糖質科学, 1, (2) 174 - 178,   2011年01月28日
  • 糖質関連酵素とバイオセンサ(複合酵素系を利用して), 宮西 伸光, 中北 愼一, 住吉 渉, 平林 淳, Journal of Applied Glycoscience Supplement, 2010,   2010年
  • Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe., Takamasa Fukunaga, Naotaka Tanaka, Toshio Furumoto, Shinichi Nakakita, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa, Glycobiology, 31, (8) 1037 - 1045,   2021年09 09
  • Structures of human galectin-10/monosaccharide complexes demonstrate potential of monosaccharides as effectors in forming Charcot-Leyden crystals., Aiko Itoh, Yasuhiro Nonaka, Shin-Ichi Nakakita, Hiromi Yoshida, Nozomu Nishi, Takanori Nakamura, Shigehiro Kamitori, Biochemical and biophysical research communications,   2020年02 17 , [Refereed]
  • Occurrence of free sialyl oligosaccharides related to N-glycans (sialyl free N-glycans) in animal sera, Junichi Seino, Haruhiko Fujihira, Shin-ichi Nakakita, Yuki Masahara-Negishi, Eiji Miyoshi, Jun Hirabayashi, Tadashi Suzuki, GLYCOBIOLOGY, 26, (10) 1072 - 1085,   2016年10 , [Refereed]
  • A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide, Yujiro Higuchi, Sho Yoshinaga, Ken-ichi Yoritsune, Hiroaki Tateno, Jun Hirabayashi, Shin-ichi Nakakita, Miho Kanekiyo, Yoshimitsu Kakuta, Kaoru Takegawa, SCIENTIFIC REPORTS, 6,   2016年05 , [Refereed]
  • Transglycosylation Activity of Glycosynthase Mutants of Endo-beta-N-Acetylglucosaminidase from Coprinopsis cinerea, Yasunari Eshima, Yujiro Higuchi, Takashi Kinoshita, Shin-Ichi Nakakita, Kaoru Takegawa, PLOS ONE, 10,   2015年07 , [Refereed]
  • Engineering of a 3 '-sulpho-Gal beta 1-4GlcNAc-specific probe by a single amino acid substitution of a fungal galectin, Dan Hu, Hang Huang, Hiroaki Tateno, Shin-ichi Nakakita, Takashi Sato, Hisashi Narimatsu, Xinsheng Yao, Jun Hirabayashi, JOURNAL OF BIOCHEMISTRY, 157, (4) 197 - 200,   2015年04 , [Refereed]
  • Improved Method for Drawing of a Glycan Map, and the First Page of Glycan Atlas, Which Is a Compilation of Glycan Maps for a Whole Organism, Shunji Natsuka, Mayumi Masuda, Wataru Sumiyoshi, Shin-ichi Nakakita, PLOS ONE, 9,   2014年07 , [Refereed]
  • Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides, Satoshi Yamashita, Hiromi Yoshida, Noboru Uchiyama, Yukari Nakakita, Shin-Ichi Nakakita, Takashi Tonozuka, Keiji Oguma, Atsushi Nishikawa, Shigehiro Kamitori, FEBS Letters, 586, (16) 2404 - 2410,   2012年07 30
  • Bovine milk whey for preparation of natural n-glycans: Structural and quantitative analysis, Nongluk Sriwilaijaroen, Sachiko Kondo, Hirokazu Yagi, Hiroaki Hiramatsu, Shin-ichi Nakakita, Keita Yamada, Hiromi Ito, Jun Hirabayashi, Hisashi Narimatsu, Koichi Kato, Yasuo Suzuki, Open Glycoscience, 5, (1) 41 - 50,   2012年, [Refereed]
  • Structural analysis of alpha 1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple alpha-galactosyltransferase genes disruptions, Takao Ohashi, Shin-ichi Nakakita, Wataru Sumiyoshi, Naotaka Yamada, Yuka Ikeda, Naotaka Tanaka, Kaoru Takegawa, GLYCOBIOLOGY, 21, (3) 340 - 351,   2011年03 , [Refereed]
  • X-ray structures of human galectin-9 C-terminal domain in complexes with a biantennary oligosaccharide and sialyllactose, Hiromi Yoshida, Misa Teraoka, Nozomu Nishi, Shin-Ichi Nakakita, Takanori Nakamura, Mitsuomi Hirashima, Shigehiro Kamitori, Journal of Biological Chemistry, 285, (47) 36969 - 36976,   2010年11 19 , [Refereed]
  • Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe, Takao Ohashi, Shin-ichi Nakakita, Wataru Sumiyoshi, Kaoru Takegawa, JOURNAL OF BIOTECHNOLOGY, 150, (3) 348 - 356,   2010年11 , [Refereed]
  • Crystallization and preliminary X-ray diffraction analysis of a protease-resistant mutant form of human galectin-8, Hiromi Yoshida, Nozomu Nishi, Shin-Ichi Nakakita, Shigehiro Kamitori, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 65, (5) 512 - 514,   2009年, [Refereed]
  • The och1 mutant of schizosaccharomyces pombe produces galactosylated core structures of n-linked oligosaccharides, Takao Ohashi, Yuka Ikeda, Naotaka Tanaka, Shin-Ichi Nakakita, Shunji Natsuka, Yuko Giga-Hama, Kaoru Takegawa, Bioscience, Biotechnology and Biochemistry, 73, (2) 407 - 414,   2009年, [Refereed]
  • Development of an amperometric flow analysis sensor for specific detection of D-psicose, Nobumitsu Miyanishi, Naruhide Sato, Shin-Ichi Nakakita, Wataru Sumiyoshi, Kenji Morimoto, Hirokazu Okuma, Masaaki Tokuda, Ken Lzumori, Etsuo Watanabe, Jun Hirabayashi, BIOSENSORS & BIOELECTRONICS, 23, (9) 1347 - 1352,   2008年04 , [Refereed]
  • A practical approach to N-glycan production by hydrazinolysis using hydrazine monohydrate, Shin-ichi Nakakita, Wataru Sumiyoshi, Nobumitsu Miyanishi, Jun Hirabayashi, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 362, (3) 639 - 645,   2007年10 , [Refereed]
  • Free oligosaccharides with lewis x structure expressed in the segmentation period of zebrafish embryo, Kaznnobu Moriguchi, Tatsuya Takemoto, Takafuini Aoki, Shin-Ichi Nakakita, Shunji Natsuka, Sumihiro Hase, Journal of Biochemistry, 142, (2) 213 - 227,   2007年08 , [Refereed]
  • Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung, Tomonori Murakami, Shunji Natsuka, Shin-Ichi Nakakita, Sumihiro Hase, Glycoconjugate Journal, 24, (4-5) 195 - 206,   2007年07 , [Refereed]
  • Gas-phase pyridylamination of saccharides: development and applications., Shin-ichi Nakakita, Wataru Sumiyoshi, Nobumitsu Miyanishi, Shunji Natsuka, Sumihiro Hase, Jun Hirabayashi, Analytical chemistry, 79, (7) 2674 - 9,   2007年04 01 , [Refereed]
  • Developmental changes in the expression of glycogenes and the content of N-glycans in the mouse cerebral cortex, Akihiro Ishii, Takeshi Ikeda, Seiji Hitoshi, Ichiro Fujimoto, Tomohiro Torii, Keiichiro Sakuma, Shin-ichi Nakakita, Sumihiro Hase, Kazuhiro Ikenaka, GLYCOBIOLOGY, 17, (3) 261 - 276,   2007年03 , [Refereed]
  • Alteration of brain type N-glycans in neurological mutant mouse brain, Shin-Ichi Nakakita, Shunji Natsuka, Jun Okamoto, Kazuhiro Ikenaka, Sumihiro Hase, Journal of Biochemistry, 138, (3) 277 - 283,   2005年09 , [Refereed]
  • Xenopus galectin-VIIa binds N-glycans of members of the cortical granule lectin family (xCGL and xCGL2), Hiroki Shoji, Kazuhiro Ikenaka, Shin-Ichi Nakakita, Koh Hayama, Jun Hirabayashi, Yoichiro Arata, Ken-Ichi Kasai, Nozomu Nishi, Takanori Nakamura, Glycobiology, 15, (7) 709 - 720,   2005年07 , [Refereed]
  • Changes in N-linked sugar chain patterns induced by moderate-to-high expression of the galactosyltransferase I gene in a brain-derived cell line, CG4, KN Menon, T Ikeda, Fujimoto, I, H Narimatsu, S Nakakita, S Hase, K Ikenaka, JOURNAL OF NEUROSCIENCE RESEARCH, 80, (1) 29 - 36,   2005年04 , [Refereed]
  • Expression of complex-type N-glycans in developmental periods of zebrafish embryo, Tatsuya Takemoto, Shunji Natsuka, Shin-Ichi Nakakita, Sumihiro Hase, Glycoconjugate Journal, 22, (1-2) 21 - 26,   2005年02 , [Refereed]
  • Conversion of pyridylamino sugar chains to corresponding reducing sugar chains, C Takahashi, S Nakakita, S Hase, JOURNAL OF BIOCHEMISTRY, 134, (1) 51 - 55,   2003年07 , [Refereed]
  • Analysis of oligosaccharide structures of glycoproteins in polyacrylamide gel, Shin-ichi Nakakita, Daisuke Ama, Shunji Natsuka, Sumihiro Hase, Analytical Biochemistry, 303, (2) 206 - 209,   2002年04 15 , [Refereed]
  • Structural analysis of N-linked glycans in Caenorhabditis elegans, Shunji Natsuka, Jiro Adachi, Masahumi Kawaguchi, Shin-Ichi Nakakita, Sumihiro Hase, Akira Ichikawa, Koji Ikura, Journal of Biochemistry, 131, (6) 807 - 813,   2002年, [Refereed]
  • Detection of tissue-specific sugar chains by two-dimensional HPLC sugar mapping of pyridylaminated sugar chains, S Nakakita, K Ikenaka, S Hase, NEW DEVELOPMENTS IN GLYCOMEDICINE, 1223,   2001年, [Refereed]
  • beta 1-4Galactosyltransferase activity of mouse brain as revealed by analysis of brain-specific complex-type N-linked sugar chains, S Nakakita, KK Menon, S Natsuka, K Ikenaka, S Hase, JOURNAL OF BIOCHEMISTRY, 126, (6) 1161 - 1169,   1999年12 , [Refereed]
  • Systematic analysis of N-linked sugar chains from whole tissue employing partial automation, Ichiro Fujimoto, Krishna K. Menon, Yosuke Otake, Fumihiro Tanaka, Hiromi Wada, Hitoshi Takahashi, Shoji Tsuji, Shunji Natsuka, Shin-Ichi Nakakita, Sumihiro Hase, Kazuhiro Ikenaka, Analytical Biochemistry, 267, (2) 336 - 343,   1999年02 15 , [Refereed]
  • Development-dependent expression of complex-type sugar chains specific to mouse brain, S Nakakita, S Natsuka, K Ikenaka, S Hase, JOURNAL OF BIOCHEMISTRY, 123, (6) 1164 - 1168,   1998年06 , [Refereed]

Misc

  • ガレクチン10・単糖複合体のX線結晶構造解析, 野中康宏, 伊藤愛子, 吉田裕美, 中北愼一, 中村隆範, 西望, 神鳥成弘, 日本生化学会大会(Web), 92nd,   2019年
  • An alternative strategy for structural glucanomics using β-gluco-oligosaccharides from the brown algae Ecklonia stolonifera as models, 住吉渉, 宮西伸光, 中北愼一, 筒井翔子, 山田佳太, 中北ゆかり, 吉岡, 浅尾正勝, 平林淳, Bioactive Carbohydrates and Dietary Fibre, 5, (2), 137 - 145,   2015年04月01日, [査読有り]
  • 脳特異的糖鎖とその生合成に関わる"脳型"ガラクトース転移酵素, 中北愼一, 長束俊治, 池中一裕, 長谷純宏, 生物物理化学 = Journal of Electrophoresis, 48, (1), 1 - 4,   2004年03月15日
  • Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species., Takamasa Fukunaga, Naotaka Tanaka, Toshio Furumoto, Shinichi Nakakita, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa, Journal of bioscience and bioengineering, 130, (2), 128 - 136,   2020 08
    The N- and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not only contain large amounts of d-mannose (Man) but also contain large amounts of d-galactose (Gal). Although the galactomannans of S. pombe are mainly composed of α1,2- or α1,3-linked Gals, some of the terminal α1,2-linked Gals are found to be linked to pyruvylated β1,3-linked galactose (PvGal). We have determined the structural characteristics of the N-glycans and O-glycans in three Schizosaccharomyces species (S. japonicus, S. octosporus, and S. cryophilus) using lectin blot, 1H NMR spectroscopy, and size-fractionation high performance liquid chromatography (HPLC), and found that the galactosylation of oligosaccharides was a common feature in fission yeasts. In addition, each of the terminal Galα1,2-, Galβ1,3- and non-substituted Man residues exhibited distinct characteristics. A BLAST search of gene databases in Schizosaccharomyces identified genes homologous to pvg1 encoding pyruvyltransferase of S. pombe. These genes, when expressed in an S. pombe pvg1Δ strains, led to the pyruvylation of non-reducing terminal β-linked Gal, suggesting the biosynthetic pathway of PvGal-containing oligosaccharides is highly conserved in fission yeasts.
  • Antigenic and Receptor Binding Properties of Enterovirus 68, Tadatsugu Imamura, Michiko Okamoto, Shin-ichi Nakakita, Akira Suzuki, Mariko Saito, Raita Tamaki, Socorro Lupisan, Chandra Nath Roy, Hiroaki Hiramatsu, Kan-etsu Sugawara, Katsumi Mizuta, Yoko Matsuzaki, Yasuo Suzuki, Hitoshi Oshitani, JOURNAL OF VIROLOGY, 88, (5), 2374 - 2384,   2014 03 , [Refereed]
    Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying molecular mechanisms for this trend are still unknown. We therefore aimed to study the antigenicity and receptor binding properties of EV68 detected in recent years in comparison to the prototype strain of EV68, the Fermon strain. We first performed neutralization (NT) and hemagglutination inhibition (HI) tests using antisera generated for EV68 strains detected in recent years. We found that the Fermon strain had lower HI and NT titers than recently detected EV68 strains. The HI and NT titers were also significantly different between strains of different genetic lineages among recently detected EV68 strains. We further studied receptor binding specificities of EV68 strains for sialyloligosaccharides using glycan array analysis. In glycan array analysis, all tested EV68 strains showed affinity for alpha 2-6-linked sialic acids (alpha 2-6 SAs) compared to alpha 2-3 SAs. Our study demonstrates that emergence of strains with different antigenicity is the possible reason for the increased detection of EV68 in recent years. Additionally, we found that EV68 preferably binds to alpha 2-6 SAs, which suggests that EV68 might have affinity for the upper respiratory tract. IMPORTANCE Numbers of cases of enterovirus 68 (EV68) infection in different parts of the world increased significantly in the late 2000s. We studied the antigenicity and receptor binding properties of recently detected EV68 strains in comparison to the prototype strain of EV68, Fermon. The hemagglutination inhibition (HI) and neutralization (NT) titers were significantly different between strains of different genetic lineages among recently detected EV68 strains. We further studied receptor binding specificities of EV68 strains for sialyloligosaccharides using glycan array analysis, which showed affinity for alpha 2-6-linked sialic acids (alpha 2-6 SAs) compared to alpha 2-3 SAs. Our study suggested that the emergence of strains with different antigenicities was the possible reason for the increased detections of EV68 in recent years. Additionally, we revealed that EV68 preferably binds to alpha 2-6 SAs. This is the first report describing the properties of EV68 receptor binding to the specific types of sialic acids.
  • Self-association of the galectin-9 C-terminal domain via the opposite surface of the sugar-binding site, Yasuhiro Nonaka, Takashi Ogawa, Souichi Oomizu, Shin-ichi Nakakita, Nozomu Nishi, Shigehiro Kamitori, Mitsuomi Hirashima, Takanori Nakamura, JOURNAL OF BIOCHEMISTRY, 153, (5), 463 - 471,   2013 05 , [Refereed]
    Galectin-9 is a lectin, which has various biological functions such as T-cell differentiation and apoptosis. Multivalency of carbohydrate binding is required for galectin-9 to function. Although galectin-1 (a proto-type galectin) forms an oligomer to obtain its multivalency, galectin-9 (a tandem-repeat-type one) has two carbohydrate recognition domains (CRD) in one polypeptide. However, a single CRD of galectin-9, especially the C-terminal one, exhibited pro-apoptotic activity suggesting oligomer formation capability. In this study, we monitored the nuclear magnetic resonance (NMR) signals of the backbone atoms of the galectin-9 C-terminal CRD (G9CCRD). Protein concentration dependence of the signals suggested that a region (F1-F4 strands) opposite to the ligand-binding site was involved in the self-association of G9CCRD. Site-directed mutagenesis in this region (Leu210, Trp277 and Leu279 to Thr; G9CCRD-3T) inhibited the self-association of G9CCRD, and improved the solubility, whereas it reduced its pro-apoptotic activity towards T cells. The high pro-apoptotic activity of G9CCRD seems to be due to the ability to form an oligomer. In addition, the same substitution in two-CRD-containing galectin-9 (G9Null-3T) also diminished the self-association and improved its solubility, although it hardly reduced the anti-proliferative and pro-apoptotic activities. G9CCRD contributes the self-association of full-length galectin-9 at high protein concentrations.
  • Development of a chemical strategy to produce rare aldohexoses from ketohexoses using 2-aminopyridine, Kayo Hasehira, Nobumitsu Miyanishi, Wataru Sumiyoshi, Jun Hirabayashi, Shin-ichi Nakakita, CARBOHYDRATE RESEARCH, 346, (17), 2693 - 2698,   2011 12 , [Refereed]
    Rare sugars are monosaccharides that are found in relatively low abundance in nature. Herein, we describe a strategy for producing rare aldohexoses from ketohexoses using the classical Lobry de Bruyn-Alberda van Ekenstein transformation. Upon Schiff-base formation of keto sugars, a fluorescence-labeling reagent, 2-aminopyridine (2-AP), was used. While acting as a base catalyst, 2-AP efficiently promoted the ketose-to-aldose transformation, and acting as a Schiff-base reagent, it effectively froze the ketose-aldose equilibrium. We could also separate a mixture of Sor, Gul, and Ido in their Schiff-base forms using a normal-phase HPLC separation system. Although Gul and Ido represent the most unstable aldohexoses, our method provides a practical way to rapidly obtain these rare aldohexoses as needed. (C) 2011 Elsevier Ltd. All rights reserved.
  • Structural analysis of N-glycans of the planarian Dugesia japonica, Shunji Natsuka, Yukiko Hirohata, Shin-ichi Nakakita, Wataru Sumiyoshi, Sumihiro Hase, FEBS JOURNAL, 278, (3), 452 - 460,   2011 02 , [Refereed]
    To investigate the relationship between phylogeny and glycan structures, we analyzed the structure of planarian N-glycans. The planarian Dugesia japonica, a member of the flatworm family, is a lower metazoan. N-glycans were prepared from whole worms by hydrazinolysis, followed by tagging with the fluorophore 2-aminopyridine at their reducing end. The labeled N-glycans were purified, and separated by three HPLC steps. By comparison with standard pyridylaminated N-glycans, it was shown that the N-glycans of planarian include high mannose-type and pauci-mannose-type glycans. However, many of the major N-glycans from planarians have novel structures, as their elution positions did not match those of the standard glycans. The results of mass spectrometry and sugar component analyses indicated that these glycans include methyl mannoses, and that the most probable linkage was 3-O-methylation. Furthermore, the methyl residues on the most abundant glycan may be attached to the non-reducing-end mannose, as the glycans were resistant to alpha-mannosidase digestion. These results indicate that methylated high-mannose-type glycans are the most abundant structure in planarians.
  • Carbohydrate-recognition domains of galectin-9 are involved in intermolecular interaction with galectin-9 itself and other members of the galectin family., Nobumitsu Miyanishi, Nozomu Nishi, Hiroko Abe, Yumiko Kashio, Rika Shinonaga, Shin-ichi Nakakita, Wataru Sumiyoshi, Akira Yamauchi, Takanori Nakamura, Mitsuomi Hirashima, Jun Hirabayashi, Glycobiology, 17, (4), 423 - 32,   2007 04
    Galectin-9 (Gal-9) is a tandem-repeat-type member of the galectin family associated with diverse biological processes, such as apoptosis, cell aggregation, and eosinophil chemoattraction. Although the detailed sugar-binding specificity of Gal-9 has been elucidated, molecular mechanisms that underlie these functions remain to be investigated. During the course of our binding study by affinity chromatography and surface plasmon resonance (SPR) analysis, we found that human Gal-9 interacts with immobilized Gal-9 in the protein-protein interaction mode. Interestingly, this intermolecular interaction strongly depended on the activity of the carbohydrate recognition domain (CRD), because the addition of potent saccharide inhibitors abolished the binding. The presence of multimers was also confirmed by Ferguson plot analysis of result of polyacrylamide gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Moreover, this intermolecular interaction was observed between Gal-9 and other galectin members, such as Gal-3 and Gal-8, but not Gal-1. Because such properties have not been reported yet, they may explain an unidentified mechanism underlying the diverse functions of Gal-9.

講演・口頭発表等

  • Characterization of N-glycans of candidate-target glycoprotein of Xenopus galectin, 第77回日本生化学会大会,   2004年

競争的資金

  • 糖鎖構造解析