Re-vascularization in diabetic retinal ischemia
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2021/04 -2024/03
Author : Suzuma Kiyoshi
【Objective】Angiopoietin-1 (Ang-1) is expressed by cells in the perivascular wall and some mesenchymal cells, and regulates vascular structural stability and angiogenesis via Tie2 receptors. Reactive oxygen species (ROS) play an important role in cell apoptosis induced by mechanical stretch stimuli. In this study, we measured and evaluated the expression of Ang-1 in a human Muller cell line in response to a mechanical stretch stimulus, and discussed its relationship to ROS.【Results】The expression of Ang-1 decreased with prolonged mechanical stretch stimulation at 10%/60 cpm, the physiological stretch frequency, for 1, 3, and 6 h, respectively. This decrease in Ang-1 expression was reversed by DPI, an NADPH oxidase inhibitor, and NAC, an ROS scavenger【Conclusion】The stretch stimulus-dependent decrease in Ang-1 expression in Muller cells may be due to increased intracellular oxidative stress caused by activation of intracellular NADPH oxidase.