Effects of neutralizing (pro)renin receptor antibody on the podocytes injury in diabetic nephropathy
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2017/08 -2019/03
Author : Rahman Md Asadur
In vitro studies were conducted to determine the protective effects of (P)RR monoclonal antibodies (mAb) in podocyte injury. The gene expression data revealed that high glucose intervention reduced the mRNA expression of nephrin compared to the osmotic control group, although the level of gene expression was very small. In contrary, (P)RR mAb treatment caused the increase expression of nephrin mRNA, suggestive of the reduced podocyte injury. In case of podocin and synaptopodin the expression level is unsatisfactory. Therefore, we tried to establish the podocyte injury model in the human immortalized podocyte, AB 8/13 cells. Gene expression of podocin, nephrin and synaptopodin in this human podocyte cell line were also not satisfactory. Therefore, the podocin data are in line with our hypothesis that (P)RR mAb has the potential to protect the podocytes against injurious effects.