MicroRNAs as early predictors of efficacy and drug resistance to molecularly targeted therapy in hepatocellular carcinoma
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2021/04 -2024/03
Author : Oura Kyoko
Sixty-six patients with uHCC treated with Atezo/Bev were included. Comparing 44 patients in the response group with 22 patients in the non-response group, 10 miRNAs were significantly elevated before treatment in the response group, especially miR-485-3p, which was higher than in the non-response group and further elevated on the next day and 3 weeks later. Serum VEGF levels before treatment were not significantly different, but both groups decreased to below detection sensitivity the next day, and the 3-week/pre-treatment ratio was significantly lower in the response group than in the non-response group. In vitro, miR-485-3p transfection suppressed migration and proliferation in HuH-7, enhanced PIAS3 expression, and suppressed STAT3/VEGF expression, which were more pronounced in cells co-cultured with HuVEC.