Utility of urine cytology as a non-invasive testing in glomerular disease.
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2015/04 -2018/03
Author : Ohsaki Hiroyuki; SOFUE Tadashi; KUSHIDA Yoshio; IRINO Satoshi; TOKUHARA Yasunori
The purpose of this study was to assess the relationship between urinary WT1-positive cells (podocytes and parietal epithelial cells) and WT1-positive cells in renal biopsy to investigate whether urinary WT1-positive cells are useful for detection of crescent formation. Fifty-two patients with kidney disease were investigated (15 cases with crescentic lesion and 37 cases with non-crescentic lesion) for immunoenzyme staining using anti-WT1 antibody for urine cytology and renal biopsy. Numbers of WT1-positive cells in urine and renal biopsy were counted, respectively. The number of urinary WT1-positive cells in patients with crescentic lesion was significantly higher than in patients with non-crescentic lesion. In addition, the best cut-off value to detect patients with crescentic lesions using urinary was 5 cells/10mL. The results of our study suggest urinary WT1-positive cells can be used to detect patients with crescentic formation using 5 cells/10mL cutoff value.