Matsuzawa Tomohiko

Researcher Information

Degree

• Ph.D（2013/03 Kyushu University）

Research funding number

• 10711971

ORCID ID

•  https://orcid.org/0000-0001-9315-5383

J-Global ID

• 202101013603321772

Research Interests

• 酵素   糖質   麹菌   酵母   キシログルカン   

Research Areas

• Life sciences / Applied biochemistry
• Life sciences / Applied microbiology
• Life sciences / Applied biochemistry

Academic & Professional Experience

• 2021/07 - Today  Kagawa UniversityFaculty of Agriculture助教
• 2017/10 - 2021/06  National Institute of Advanced Industrial Science and Technology生物プロセス研究部門主任研究員
• 2013/04 - 2017/09  National Institute of Advanced Industrial Science and Technology生物プロセス研究部門研究員

Education

• 2010/04 - 2013/03  Kyushu University  Graduate School of Bioresource and Bioenvironmental Sciences  生命機能科学
• 2008/04 - 2010/03  Kagawa University  Graduate School of Agriculture  生物資源利用学
• 2004/04 - 2008/03  Kagawa University  Faculty of Agriculture  生命機能科学科

Association Memberships

• 日本応用糖質科学会   日本生物工学会   日本農芸化学会   

Published Papers

• Dilution effect of TEMPO-oxidized cellulose nanofibers on reproduction of Daphnia magna.

  Hiroyuki Mano; Rie Tai; Akihiro Moriyama; Yoko Iizumi; Tomohiko Matsuzawa; Toshiya Okazaki; Isamu Ogura

  Ecotoxicology and Environmental Safety 294 118101  2025/04 [Refereed]
  
• 糸状菌が生産する多糖類分解酵素に関する研究

  松沢智彦

  応用糖質科学 15 (1) 12 - 17 2025/03 [Invited]
  
• Production of Isoprimeverose from Xyloglucan Using Aspergillus oryzae

  Tomohiko Matsuzawa; Naoki Shimada; Shimma Fujiwa

  Journal of Applied Glycoscience The Japanese Society of Applied Glycoscience 72 7201202  1344-7882 2025/02 [Refereed]
  
• Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium

  Yutaka Nakazawa; Masumi Kageyama; Tomohiko Matsuzawa; Ziqin Liang; Kaito Kobayashi; Hisaka Shimizu; Kazuki Maeda; Miho Masuhiro; Sei Motouchi; Saika Kumano; Nobukiyo Tanaka; Kouji Kuramochi; Hiroyuki Nakai; Hayao Taguchi; Masahiro Nakajima

  Communications Biology Springer Science and Business Media LLC 8 (1) 66  2399-3642 2025/01 [Refereed]
  
• 植物の多糖類と黄麹菌の分解酵素

  松沢智彦

  日本醸造協会誌 119 (12) 652 - 658 2024/12 [Refereed][Invited]
  
• 黄麹菌由来キシログルカン分解酵素の機能と構造

  松沢智彦; 渡邊真宏

  生化学 公益社団法人生化学会 96 (5) 690 - 694 2024/10 [Refereed][Invited]
  
• Identification and characterization of xyloglucan-degradation related α-1,2-l-fucosidase in Aspergillus oryzae

  Naoki Shimada; Akihiko Kameyama; Masahiro Watanabe; Takehiko Sahara; Tomohiko Matsuzawa

  Journal of Bioscience and Bioengineering Elsevier BV 138 (3) 196 - 205 1389-1723 2024/09 [Refereed]
  
• Applicability of enzymatic and phenol–sulfuric acid methods for determination of cellulose nanofibers in ecotoxicity testing

  Tomohiko Matsuzawa; Rie Tai; Hiroyuki Mano; Isamu Ogura

  Journal of Wood Science Springer Science and Business Media LLC 70 (1) 17  1611-4663 2024/04 [Refereed]
  
• Plant polysaccharide degradation-related enzymes in Aspergillus oryzae

  Tomohiko Matsuzawa

  Bioscience, Biotechnology, and Biochemistry Oxford University Press (OUP) 88 (3) 276 - 282 1347-6947 2024/02 [Refereed][Invited]
  
• Identification and characterization of novel intracellular α-xylosidase in Aspergillus oryzae

  Tomohiko Matsuzawa, Yusuke Nakamichi, Naoki Shimada

  Journal of Applied Glycoscience 日本応用糖質科学会 70 (4) 119 - 125 2023/12 [Refereed]
  
• Optimization of microplate-based phenol-sulfuric acid method and application to the multi-sample measurements of cellulose nanofibers

  Isamu Ogura; Miyuki Sugiyama; Rie Tai; Hiroyuki Mano; Tomohiko Matsuzawa

  Analytical Biochemistry Elsevier BV 681 115329  0003-2697 2023/09 [Refereed]
  
• 麹菌のキシログルカン分解戦略

  松沢智彦

  応用糖質科学 日本応用糖質科学会 13 (2) 117 - 123 2185-6427 2023/08 [Refereed][Invited]
  
• 植物の多糖類を分解する微生物の緻密な酵素システム

  松沢智彦

  生物工学会誌 日本生物工学会 101 (4) 183 - 186 2023/04 [Refereed][Invited]
  
• LsSpt23p is a regulator of triacylglycerol synthesis in the oleaginous yeast Lipomyces starkeyi

  Hiroaki Takaku; Haruka Kazama; Rikako Sato; Kazuki Mori; Satoshi Ara; Koji Ishiya; Tomohiko Matsuzawa; Katsuro Yaoi; Hideo Araki; Yosuke Shida; Wataru Ogasawara; Kosuke Tashiro; Satoru Kuhara; Harutake Yamazaki; Sachiyo Aburatani

  Applied Microbiology and Biotechnology Springer Science and Business Media LLC 107 (4) 1269 - 1284 0175-7598 2023/01 [Refereed]
  
• Structural basis for the catalytic mechanism of the glycoside hydrolase family 3 isoprimeverose‐producing oligoxyloglucan hydrolase from Aspergillus oryzae

  Tomohiko Matsuzawa; Masahiro Watanabe; Yusuke Nakamichi; Hironaga Akita; Katsuro Yaoi

  FEBS Letters Wiley 0014-5793 2022/06 [Refereed]
  
• Crystal structure of glycoside hydrolase family 31 α-xylosidase from a soil metagenome

  Yusuke Nakamichi; Tomohiko Matsuzawa; Masahiro Watanabe; Katsuro Yaoi

  Bioscience, Biotechnology, and Biochemistry Informa UK Limited 2022/06 [Refereed]
  
• Crystal structure of metagenomic β-glycosidase MeBglD2 in complex with various saccharides

  Tomohiko Matsuzawa; Masahiro Watanabe; Yusuke Nakamichi; Hironaga Akita; Katsuro Yaoi

  Applied Microbiology and Biotechnology Springer Science and Business Media LLC 0175-7598 2022/06 [Refereed]
  
• Discovery by metagenomics of a functional tandem repeat sequence that controls gene expression in bacteria

  Hikaru Suenaga; Tomohiko Matsuzawa; Takehiko Sahara

  FEMS Microbiology Ecology Oxford University Press (OUP) 98 (4) 2022/04 [Refereed]
  
• Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.

  Tomohiko Matsuzawa; Masahiro Watanabe; Yusuke Nakamichi; Akihiko Kameyama; Naoshi Kojima; Katsuro Yaoi

  Applied Microbiology and Biotechnology 106 (2) 675 - 687 2022/01 [Refereed]
  
• Isolation and characterization of Lipomyces starkeyi mutants with greatly increased lipid productivity following UV irradiation.

  Hiroaki Takaku; Sayaka Ebina; Kotoha Kasuga; Rikako Sato; Satoshi Ara; Haruka Kazama; Tomohiko Matsuzawa; Katsuro Yaoi; Hideo Araki; Yosuke Shida; Wataru Ogasawara; Koji Ishiya; Sachiyo Aburatani; Harutake Yamazaki

  Journal of Bioscience and Bioengineering 131 (6) 613 - 621 2021/06 [Refereed]
  
• Enzymatic degradation of xyloglucans by Aspergillus species: a comparative view of this genus.

  Tomohiko Matsuzawa; Akira Watanabe; Takahiro Shintani; Katsuya Gomi; Katsuro Yaoi

  Applied Microbiology and Biotechnology 105 (7) 2701 - 2711 2021/04 [Refereed]
  
• Identification and characterization of Pseudozyma antarctica Δ12 fatty acid desaturase and its utilization for the production of polyunsaturated fatty acids.

  Tomohiko Matsuzawa; Tomoko Maehara; Yasushi Kamisaka; Yuko Ayabe-Chujo; Hiroaki Takaku; Katsuro Yaoi

  Journal of Bioscience and Bioengineering 130 (6) 604 - 609 2020/12 [Refereed]
  
• Identification and characterization of two xyloglucan-specific endo-1,4-glucanases in Aspergillus oryzae.

  Tomohiko Matsuzawa; Akihiko Kameyama; Yusuke Nakamichi; Katsuro Yaoi

  Applied Microbiology and Biotechnology 104 (20) 8761 - 8773 2020/10 [Refereed]
  
• Lipid metabolism of the oleaginous yeast Lipomyces starkeyi.

  Hiroaki Takaku; Tomohiko Matsuzawa; Katsuro Yaoi; Harutake Yamazaki

  Applied Microbiology and Biotechnology 104 (14) 6141 - 6148 2020/07 [Refereed]
  
• Identification and characterization of two fatty acid elongases in Lipomyces starkeyi.

  Tomohiko Matsuzawa; Yasushi Kamisaka; Tomoko Maehara; Hiroaki Takaku; Katsuro Yaoi

  Applied Microbiology and Biotechnology 104 (6) 2537 - 2544 2020/03 [Refereed]
  
• A novel electroporation procedure for highly efficient transformation of Lipomyces starkeyi.

  Hiroaki Takaku; Atsumi Miyajima; Haruka Kazama; Rikako Sato; Satoshi Ara; Tomohiko Matsuzawa; Katsuro Yaoi; Hideo Araki; Yosuke Shida; Wataru Ogasawara; Harutake Yamazaki

  Journal of Microbiological Methods 169 105816 - 105816 2020/02 [Refereed]
  
• Identification and characterization of α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.

  Tomohiko Matsuzawa; Akihiko Kameyama; Katsuro Yaoi

  Applied Microbiology and Biotechnology 104 (1) 201 - 210 2020/01 [Refereed]
  
• Selective fluorescence labeling: time-lapse enzyme visualization during sugarcane hydrolysis

  Makiko Imai; Asako Mihashi; Tomoya Imai; Satoshi Kimura; Tomohiko Matsuzawa; Katsuro Yaoi; Nozomu Shibata; Hiroshi Kakeshita; Kazuaki Igarashi; Yoshinori Kobayashi; Junji Sugiyama

  Journal of Wood Science Springer Science and Business Media LLC 65 (1) 1435-0211 2019/12 [Refereed]
  
• Cooperation between β-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae.

  Tomohiko Matsuzawa; Masahiro Watanabe; Tomoshi Kameda; Akihiko Kameyama; Katsuro Yaoi

  FEBS Journal 286 (16) 3182 - 3193 2019/08 [Refereed]
  
• The Metagenome Approach: A New Resource for Glycosidases

  Tomohiko Matsuzawa

  Trends in Glycoscience and Glycotechnology Forum: Carbohydrates Coming of Age 31 (178) E15 - E20 0915-7352 2019/01 [Refereed]
  
• Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme.

  Tomohiko Matsuzawa; Masahiro Watanabe; Yusuke Nakamichi; Zui Fujimoto; Katsuro Yaoi

  Journal of Structural Biology 205 (1) 84 - 90 2019/01 [Refereed]
  
• A novel isoprimeverose-producing enzyme from Phaeoacremonium minimum is active with low concentrations of xyloglucan oligosaccharides.

  Tomohiko Matsuzawa; Akihiko Kameyama; Katsuro Yaoi

  FEBS Open Bio 9 (1) 92 - 100 2019/01 [Refereed]
  
• Rational protein design for thermostabilization of glycoside hydrolases based on structural analysis.

  Masahiro Watanabe; Tomohiko Matsuzawa; Katsuro Yaoi

  Applied Microbiology and Biotechnology 102 (20) 8677 - 8684 2018/10 [Refereed]
  
• Identification and characterization of Δ12 and Δ12/Δ15 bifunctional fatty acid desaturases in the oleaginous yeast Lipomyces starkeyi.

  Tomohiko Matsuzawa; Tomoko Maehara; Yasushi Kamisaka; Satoshi Ara; Hiroaki Takaku; Katsuro Yaoi

  Applied Microbiology and Biotechnology 102 (20) 8817 - 8826 2018/10 [Refereed]
  
• Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its X-ray crystal structure.

  Tomohiko Matsuzawa; Masahiro Watanabe; Katsuro Yaoi

  Applied Microbiology and Biotechnology 101 (23-24) 8353 - 8363 2017/12 [Refereed]
  
• Crystal structure of metagenomic β-xylosidase/ α-l-arabinofuranosidase activated by calcium.

  Tomohiko Matsuzawa; Satoshi Kaneko; Naomi Kishine; Zui Fujimoto; Katsuro Yaoi

  Journal of Biochemistry 162 (3) 173 - 181 2017/09 [Refereed]
  
• Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library.

  Tomohiko Matsuzawa; Katsuro Yaoi

  Applied Microbiology and Biotechnology 101 (2) 633 - 646 2017/01 [Refereed]
  
• Screening, identification, and characterization of α-xylosidase from a soil metagenome.

  Tomohiko Matsuzawa; Nobutada Kimura; Hikaru Suenaga; Katsuro Yaoi

  Journal of Bioscience and Bioengineering 122 (4) 393 - 9 2016/10 [Refereed]
  
• Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates.

  Tomohiko Matsuzawa; Satoshi Kaneko; Katsuro Yaoi

  Applied Microbiology and Biotechnology 100 (18) 8043 - 51 2016/09 [Refereed]
  
• Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic β-glucosidase Td2F2.

  Tomohiko Matsuzawa; Toshinori Jo; Taku Uchiyama; Jenny A Manninen; Takatoshi Arakawa; Kentaro Miyazaki; Shinya Fushinobu; Katsuro Yaoi

  FEBS Journal 283 (12) 2340 - 53 2016/06 [Refereed]
  
• Identification of the Gene Encoding Isoprimeverose-producing Oligoxyloglucan Hydrolase in Aspergillus oryzae.

  Tomohiko Matsuzawa; Yasushi Mitsuishi; Akihiko Kameyama; Katsuro Yaoi

  Journal of Biological Chemistry 291 (10) 5080 - 7 2016/03 [Refereed]
  
• GH74 Xyloglucanases: Structures and Modes of Activity

  Tomohiko Matsuzawa; Katsuro Yaoi

  Trends in Glycoscience and Glycotechnology Forum: Carbohydrates Coming of Age 28 (162) E63 - E70 0915-7352 2016 [Refereed]
  
• Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome.

  Tomohiko Matsuzawa; Satoshi Kaneko; Katsuro Yaoi

  Applied Microbiology and Biotechnology 99 (21) 8943 - 54 2015/11 [Refereed]
  
• Draft Genome Sequence of the Yeast Starmerella bombicola NBRC10243, a Producer of Sophorolipids, Glycolipid Biosurfactants.

  Tomohiko Matsuzawa; Hideaki Koike; Azusa Saika; Tokuma Fukuoka; Shun Sato; Hiroshi Habe; Dai Kitamoto; Tomotake Morita

  Genome Announcements 3 (2) 2015/03 [Refereed]
  
• The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a Trichoderma reesei mutant.

  Yosuke Shida; Kaori Yamaguchi; Mikiko Nitta; Ayana Nakamura; Machiko Takahashi; Shun-Ichi Kidokoro; Kazuki Mori; Kosuke Tashiro; Satoru Kuhara; Tomohiko Matsuzawa; Katsuro Yaoi; Yasumitsu Sakamoto; Nobutada Tanaka; Yasushi Morikawa; Wataru Ogasawara

  Biotechnology for Biofuels 8 230 - 230 2015 [Refereed]
  
• Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe.

  Ken-ichi Yoritsune; Yujiro Higuchi; Tomohiko Matsuzawa; Kaoru Takegawa

  FEMS Yeast Research 14 (7) 1101 - 9 2014/11 [Refereed]
  
• Key amino acid residues for the endo-processive activity of GH74 xyloglucanase.

  Tomohiko Matsuzawa; Yuji Saito; Katsuro Yaoi

  FEBS Letters 588 (9) 1731 - 8 2014/05 [Refereed]
  
• Ethanol-inducible gene expression using gld1 (+) promoter in the fission yeast Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Hideki Tohda; Kaoru Takegawa

  Applied microbiology and biotechnology 97 (15) 6835 - 43 2013/08 [Refereed]
  
• Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Futoshi Hara; Naotaka Tanaka; Hideki Tohda; Kaoru Takegawa

  Applied Microbiology and Biotechnology 97 (11) 4957 - 64 2013/06 [Refereed]
  
• The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast.

  Tomohiko Matsuzawa; Youko Kageyama; Kazuyoshi Ooishi; Makoto Kawamukai; Kaoru Takegawa

  FEMS Yeast Research 13 (3) 259 - 66 2013/05 [Refereed]
  
• The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.

  Ken-ichi Yoritsune; Tomohiko Matsuzawa; Takao Ohashi; Kaoru Takegawa

  FEBS Letters 587 (7) 917 - 21 2013/04 [Refereed]
  
• Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Futoshi Hara; Hideki Tohda; Hiroshi Uemura; Kaoru Takegawa

  Applied Microbiology and Biotechnology 95 (2) 441 - 9 2012/07 [Refereed]
  
• Genome sequence of Pantoea agglomerans strain IG1.

  Tomohiko Matsuzawa; Kazuki Mori; Takeshi Kadowaki; Misato Shimada; Kosuke Tashiro; Satoru Kuhara; Hiroyuki Inagawa; Gen-ichiro Soma; Kaoru Takegawa

  Journal of Bacteriology 194 (5) 1258 - 9 2012/03 [Refereed]
  
• Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Yasuko Fujita; Hideki Tohda; Kaoru Takegawa

  Eukaryotic Cell 11 (2) 159 - 67 2012/02 [Refereed]
  
• MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast.

  Tomohiko Matsuzawa; Ken-ichi Yoritsune; Kaoru Takegawa

  Eukaryotic Cell 11 (2) 151 - 8 2012/02 [Refereed]
  
• Galactose-Specific Recognition System in the Fission Yeast Schizosaccharomyces pombe

  Matsuzawa Tomohiko; Ohashi Takao; Nakase Mai; Yoritsune Ken-ichi; Takegawa Kaoru

  Trends in Glycoscience and Glycotechnology FCCA(Forum: Carbohydrates Coming of Age) 24 (135) 24 - 42 2012
• Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Tomotake Morita; Naotaka Tanaka; Hideki Tohda; Kaoru Takegawa

  Molecular Microbiology 82 (6) 1531 - 44 2011/12 [Refereed]
  
• New insights into galactose metabolism by Schizosaccharomyces pombe: isolation and characterization of a galactose-assimilating mutant.

  Tomohiko Matsuzawa; Yasuko Fujita; Naotaka Tanaka; Hideki Tohda; Akiko Itadani; Kaoru Takegawa

  Journal of Bioscience and Bioengineering 111 (2) 158 - 66 2011/02 [Refereed]
  
• Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis.

  Tomoko Hirano; Tomohiko Matsuzawa; Kaoru Takegawa; Masa H Sato

  Plant Physiology 155 (2) 797 - 807 2011/02 [Refereed]
  
• The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe.

  Tomohiko Matsuzawa; Takao Ohashi; Akira Hosomi; Naotaka Tanaka; Hideki Tohda; Kaoru Takegawa

  Applied Microbiology and Biotechnology 87 (2) 715 - 27 2010/06 [Refereed]
  
• Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast.

  Shotaro Suzuki; Tomohiko Matsuzawa; Yayoi Nukigi; Kaoru Takegawa; Naotaka Tanaka

  Microbiology 156 (Pt 3) 708 - 718 2010/03 [Refereed]
  

MISC

• メタゲノムを利用した有用酵素の探索

  松沢智彦; 木村信忠; 末永光; 末永光  応用糖質科学  8-  (2)  2018
• 油脂酵母Lipomyces starkeyiのdelta12-fatty-acid desaturaseの機能解析

  松沢智彦; 前原智子; 神坂泰; 荒学志; 高久洋暁; 矢追克郎  日本農芸化学会大会講演要旨集(Web)  2018-  2018
• メタゲノムを活用した新規ホスホリラーゼスクリーニング法の構築

  柴田理沙; 佐藤耕平; 杉本直久; 仁平高則; 北岡本光; 松沢智彦; 末永光; 矢追克郎; 片山高嶺; 中井博之  応用糖質科学  7-  (3)  2017
• Bp-8 進化分子工学によるTrichoderma reesei由来キシラナーゼIII(XynIII)の耐熱化(キシラン、セルロース、グルカン関連酵素,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

  矢追 克郎; 松沢 智彦  応用糖質科学 : 日本応用糖質科学会誌  5-  (3)  B44  2015/08
• Bp-9 バイオマス糖化を促進するメタゲノム由来GH43キシロシダーゼCoXyl43の解析(キシラン、セルロース、グルカン関連酵素,一般講演,一般社団法人日本応用糖質科学会平成27年度大会(第64回))

  松沢 智彦; 金子 哲; 矢追 克郎  応用糖質科学 : 日本応用糖質科学会誌  5-  (3)  B45  2015/08
• 2P-027 Enhancement of thermostability of Trichoderma reesei XynIII

  Matsuzawa Tomohiko; Yaoi Katsurou  日本生物工学会大会講演要旨集  67-  181  -181  2015
• 3P-161 Isolation and characterization of GH43 xylosidase from a compost metagenome

  Matsuzawa Tomohiko; Kaneko Satoshi; Yaoi Katsuro  日本生物工学会大会講演要旨集  66-  235  -235  2014
• 酵母のストレス逃避術(バイオミディア)

  松沢 智彦  生物工学会誌 : seibutsu-kogaku kaishi  91-  (9)  528  -528  2013
• 3Dp04 Identification of a ethanol-inducible promoter from fission yeast and its use as an expression vector.

  Matsuzawa Tomohiko; Takegawa Kaoru  日本生物工学会大会講演要旨集  64-  133  -133  2012

Awards & Honors

• 2024/09 日本応用糖質科学会 奨励賞
  
• 2023/03 日本農芸化学会 農芸化学奨励賞
  
• 2022/10 日本生物工学会 若手賞
  
• 2019/01 日本木質学会 Journal of Wood Science論文賞
  
• 2017/09 日本応用糖質科学会東日本支部 奨励賞
  
• 2013/01 日本農芸化学会西日本支部 奨励賞（学生の部）
  
• 2012/12 日本生物工学会九州支部 学生賞
  
• 2008/03 日本農芸化学会中四国支部 学生奨励賞
  

Research Grants & Projects

• 植物の多糖類の構造的多様性に適応した麹菌の分解システムの解析

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2024/04 -2028/03 

  Author : 松沢 智彦
• 瀬戸内海の物質循環を協調的に支える微生物群の解明

  公益財団法人 発酵研究所：研究室助成

  Date (from‐to) : 2023/04 -2027/03
• オミックス解析による酵素の探索とその理解

  公益財団法人 発酵研究所：一般研究室助成

  Date (from‐to) : 2022/04 -2024/03
• 微生物のオリゴ糖を介した環境認識

  日本学術振興会：科学研究費助成事業 基盤研究(B)

  Date (from‐to) : 2018/04 -2022/03 

  Author : 松沢 智彦

   

  麹菌Aspergillus oryzaeがオリゴ糖存在下において発現を誘導する推定糖質分解酵素の機能解析を進め、新たに植物由来多糖類（キシログルカン）の分解に重要な酵素（α-キシロシダーゼ）を見いだすことができた。本酵素はこれまでの研究において同定したイソプリメベロース（グルコースとキシロースから成る二糖）生成酵素によって生成されたイソプリメベロースを分解する酵素であり、イソプリメベロース以外にもキシログルカンオリゴ糖のキシロース側鎖にも作用することが明らかになった。本酵素以外にも、多糖類をオリゴ糖化する複数の糖質加水分解酵素を同定することができた。また、昨年度までに見出したオリゴ糖依存的に発現が誘導される酵素をコードする遺伝子を破壊した麹菌の作製を行った。 昨年度までに単離したユニークな酵素について結晶構造解析を進め、その立体構造情報を基に、複数の変異酵素の取得およびその酵素学的諸性質の解析によって酵素の基質認識メカニズムなどの解析を進めた。 昨年度までに行ったトランスクリプトーム解析の結果を参考に、新たな培養条件での遺伝子発現解析（リアルタイムPCR解析など）を進めた。また、上記酵素をコードする遺伝子の転写制御機構を明らかにするために、Yeast One Hybrid法による当該遺伝子の推定プロモーター領域に結合する転写因子を探索するための基盤構築を進めた。具体的には、レポーターアッセイに使用するプロモーター領域の検討や複数の推定転写因子のクローニングなどを進めた。
• 新規有用物質生産に資する麹菌酵素ライブラリーの構築と利用

  公益財団法人 野田産業科学研究所：奨励研究助成

  Date (from‐to) : 2020/04 -2021/03
• Analysis and applications of metagenomic high-performance beta-glucosidases

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

  Date (from‐to) : 2014/04 -2018/03 

  Author : Matsuzawa Tomohiko

   

  Novel β-glucosidases, which showed tolerance to product-inhibition and high temperature, were isolated from environmental metagenomes. A novel β-glucosidase showed unique enzymatic properties and had positive effects on the enzymatic saccharification of lignocellulosic biomass. In addition, thermostability of the β-glucosidase was improved by site-directed mutagenesis.
• 糖類の代謝経路を改変した酵母による有用物質生産系の確立

  日本学術振興会：科学研究費助成事業 特別研究員奨励費

  Date (from‐to) : 2011 -2012 

  Author : 松沢 智彦

   

  微生物による物質生産には強力な遺伝子発現誘導システムが不可欠である。私は分裂酵母のグルコース飢餓応答機構やグリセロール代謝の制御機構の解明、並びにこれらの改変によるグリセロールを資源として用いた物質生産を目指し、研究を行っている。前年度までの研究によって分裂酵母のグルコース飢餓応答機構やグリセロール代謝の制御機構を明らかにすることができた。そこで、次に、グリセロールを資源とした培地において機能する強力な遺伝子発現誘導システムの構築を行った。グリセロールを炭素源・エネルギー源として利用する(資化)する為に必須なグリセロールデヒドロゲナーゼをコードするgld1遺伝子のプロモーター領域を用い、遺伝子発現誘導システムの構築を行った。gld1遺伝子はグルコースが豊富な条件下では発現が抑制され、グリセロール培地にエタノールや1- ガラクトースの代謝経路とその制御メカニズムを解明するにあたり、ガラクトース代謝の最初のステップであるガラクトースの細胞外から細胞内への取り込みに魚要であると考えられるヘキソーストランスポーターGht2を同定した。Ght2はグルコース豊富な条件下においてガラクトースの取り込みに関与していると考えられる。

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https://researchmap.jp/matsuzawatomohiko