The elucidation of the physiologic function of rare sugar and the exploitation of prophylactic drug for necrotizing enterocolitis
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2017/04 -2021/03
Author : Shimono Ryuichi
We investigated whether PHGG ameliorates small intestinal mucosal damage and alters the intestinal microbiota using a rat small bowel resection (SBR) model. Sprague Dawley rats were divided into sham operation (Sham), Sham/PHGG, SBR, and SBR/PHGG groups. On day 21, all rats were euthanized. To assess small intestinal mucosal damage, the degeneration rate was morphometrically evaluated and immunohistochemically examined using anti-CD45 antibodies. Analyses of fecal microbiota using 16S rRNA and short-chain fatty acid production were also performed. The mucosal degeneration rate was significantly higher in the SBR group than in the Sham or SBR/PHGG groups. The number of CD45-positive cells was significantly higher in the SBR group than in the Sham, Sham/PHGG, or SBR/PHGG groups. PHGG administration alleviated small intestinal mucosal damage which could be associated with modulation of the intestinal microbiota.