Mechanisms for switching off of CaM-kinases and their involvement in regulation of the cellular phosphorylation balance
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2009 -2011
Author : ATSUHIKO Ishida; TAKESHI Yamazaki; TATSUO Nehira; TAKAO Mukuda; ISAMU Kameshita; NORIYUKI Sueyoshi
To clarify intracellular dynamics and physiological significance of CaM-kinase phosphatse(CaMKP/ PPM1F) and its nuclear homolog, CaMKP-N(PPM1E), which had been identified as protein phosphatases involved in negative regulation of multifunctional CaM-kinases, we carried out functional analyses of these enzymes using zebrafish as a model system. These in vitro and in vivo studies revealed that CaMKP plays a pivotal role in embryogenesis of zebrafish, and that processing of CaMKP-N regulates its catalytic activity and subcellular localization.