Study on the production of rare disaccharides and rare sugars.
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Date (from‐to) : 2014/04 -2017/03
Author : Takata Goro; Sakuraba Haruhiko
The objective of this study is the production of D-allose, D-altrose and their disaccharides using biotechnology method.
From the result of this research, we succeeded to mass-production of recombinant D-glucoside 3-dehydrogenase and its crystalization. This enzyme showed the substrate specificity against various disaccharides. By combination of chemical hydrogenation, we achieved to produced various rare sugar containing disaccharide such as 1,4-a-allosylglucose, 1,4-b-allosylglucose, 1,1-a-allosylallose, and 1,4-b-gulosylglucose.