Molecular Mechanisms of D-Amino Acid Signaling: Elucidation and Development
Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
Date (from‐to) : 2019/04 -2022/03
Author : Yoshimura Tohru
We demonstrated that (1) serine racemase (SerR) catalyzes the dehydration of D-Ser without its racemization, (2) the PLP-binding lysine residue of mouse SerR was reacted with the substrate and converted to lysinoalanine, resulting in an increase in D-Ser dehydratase activity, and (3) administration of yeast D-serine dehydratase modified with polyethylene glycol reduces D-Ser levels in the spinal cord and blood of mice. We also attained that (4) identification of the gene for silkworm SerR and its enzymatic properties, (5) elucidation of the mechanism of Ser racemization by D-amino acid transaminase, (6) establishment of a functional screening method for D-amino acid biosynthetic enzyme genes, and (7) elucidation of the reaction mechanism of D-amino acid N-acetyltransferase.