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Hirano Katsuya

  • Faculty of Medicine
  • School of Medicine
  • International Instutute of Rare Sugar Research and Education
  • Professor
Last Updated :2025/04/19

Researcher Information

Degree

  • M. D., Ph.D.（1991/07 Kyushu University）

Research funding number

  • 80291516

ORCID ID

Researcher ID

  • B-2785-2008

J-Global ID

Research Interests

  • Proteinase-activated receptor   Blood coagulation facotr   Aging   Physiology   Pathophysiology   Intracellular signal tranduction   Circulatory system   Vascular endothelial cell   Smooth muscle   

Research Areas

  • Life sciences / Nutrition and health science / Aging
  • Life sciences / Physiology
  • Life sciences / Pharmacology
  • Life sciences / Cardiology

Academic & Professional Experience

  • 2014/04  Kagawa University, Faculty of MedicineDepartment of Cardiovascular PhysiologyProfessor
  • 2006/08 - 2014/03  Kyushu University, Graduate School of MedicineDepartment of Molecular CardiologyAssociate Professor
  • 2000/04 - 2006/07  Kyushu University, Graduate School of MedicineDepartment of Molecular CardiologyAssistant professor, Lecturer
  • 1999/04 - 2000/03  Kyushu University, Graduate School of MedicineDepartment of Molecular CardiologyAssistant professor, Lecturer
  • 1996/11 - 1999/03  Kyushu University, Faculty of MedicineDepartment of Molecular CardiologyAssistant professor, Lecturer
  • 1993/09 - 1996/11  University of Arizona, Tucson, AZ, USADepartment of Physiology, Biochemistry, Animal Sciences; Muscle Biology GroupAssistant Research Scientist
  • 1990/10 - 1993/08  University of Arizona, Tucson, AZ, USADepartment of Physiology, Biochemistry, Animal Sciences; Muscle Biology GroupResearch Associate
  • 1987/06 - 1990/10  Kyushu University, Graduate School of MedicineDepartment of Molecular CardiologyResearch fellow (thesis work)
  • 1985/06 - 1987/05  Kyushu University HospticalDepartment of Cardiovascular MedicineResident

Education

  • 1991/07 -   Kyushu University  医学研究院 医学博士
  • 1979/04 - 1985/03  Kyushu University  Facultyof Medicine  School of Medicine

Association Memberships

  • 日本循環薬理学会   日本病態生理学会   国際心臓研究学会   日本平滑筋学会   日本心脈管作動物質学会   日本生理学会   日本分子生物学会   日本内科学会   日本循環器学会   American Heart Association   日本薬理学会   

Published Papers

Books etc

  • The unique property of the pulmonary artery regarding the smooth muscle effects of proteinase-activated receptor 1: The possible contribution to the pathogenesis of pulmonary hypertension.
    Springer, Tokyo, Japan 2017
  • The unique property of the pulmonary artery regarding the smooth muscle effects of proteinase-activated receptor 1: The possible contribution to the pathogenesis of pulmonary hypertension.
    Springer, Tokyo, Japan 2017
  • Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry.
    Humana Press, New York, 2013 9781627030854
  • Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry.
    Humana Press, New York, 2013 9781627030854
  • Idendification of hydrogen peroxide as an endothelium-derived hyperpolarization factor in mice.
    Taylor & Francis, London, UK 2001
  • Cytosolic calcium transients in vascular smooth muscle.
    in Ion Channels of Vascular Smooth Muscle Cells and Endothelial Cells, eds Sperelakis N and Kuriyama H., Elsivier, New York, 1991
  • Effects of okadaic acid on cytosolic calcium concentration and contraction of porcine coronary artery.
    in Advances in Second Messenger and Phosphoprotein Research vol.24, ed. Nishizuka Y., Raven Press, New York, 1990
  • Effecs of okadaic acid on cytosolic calcium concentration and contraction of porcine coronary arter
    Raven Press, New York 1990

Conference Activities & Talks

  • Novel role of coagulation factor XI as a regulator of vascular smooth muscle function.  [Not invited]
    HIRANO Katsuya
    The 9th Federation of the Asian and Oceanian Physiological Society Congress (FAOPS2019)  2019/03
  • Proteinase-activated receptor 1 (PAR1)-mediated cellular effects of coagulation factor XI in vascular smooth muscle cells  [Not invited]
    HIRANO Katsuya
    第92回日本薬理学会年会  2019/03
  • 血管平滑筋細胞において凝固第XI因子はトロンビン受容体PAR1を活性化し、細胞内Ca2+シグナルを発生させ、細胞遊走を促進する  [Not invited]
    平野 勝也
    第48回日本心脈管作動物質学会総会  2019/02
  • トロンビンによる内皮バリアー機能障害の初期事象におけるRho-Rhoキナーゼの役割  [Not invited]
    平野 勝也
    第59回日本脈管学会総会  2018/10
  • Novel function of coagulation factor XI as a regulator of vascular smooth muscle  [Not invited]
    Hirano K; Liu W; Hashimoto T; Yamashita T
    The 7th Chiang Mai University-Kagawa University Joint Symposium 2018  2018/08
  • 凝固第XI因子の血管平滑筋作用  [Not invited]
    平野 勝也
    第３回日本肺高血圧・肺循環学会学術集会  2018/06
  • 血液凝固第XI因子は電位作動性Ca2+チャネルを介して血管平滑筋細胞にCa2+シグナルを発生させる  [Not invited]
    第47回日本心脈管作動物質学会年会  2018
  • Coagulaton factor XI induced Ca2+ response and cell migration in vascular smooth muscle cells  [Not invited]
    第95回日本生理学会大会  2018
  • Novel strategy for treatment of pulmonary hypertension based on the unique property of pulmonary circulation regarding smooth muscle effect of proteinase-activated receptor 1  [Not invited]
    第95回日本生理学会大会  2018
  • 血管収縮の生理学‐平滑筋収縮装置のカルシウム感受性制御機構を中心に  [Not invited]
    第95回日本生理学会大会  2018
  • Coagulaton factor XI induced Ca2+ response and cell migration in vascular smooth muscle cells  [Not invited]
    第95回日本生理学会大会  2018
  • Novel strategy for treatment of pulmonary hypertension based on the unique property of pulmonary circulation regarding smooth muscle effect of proteinase-activated receptor 1  [Not invited]
    第95回日本生理学会大会  2018
  • Physiology of vasoconstriction with a focus on the regulation of Ca2+ sensitivity of the contractile apparatus of smooth muscle  [Not invited]
    第95回日本生理学会大会  2018
  • トロンビン受容体を標的とする新規肺高血圧治療の提案  [Not invited]
    第46回日本心脈管作動物質学会  2017
  • A novel angiogenic agent COA-Cl promotes VEGF secretion from mouse C2C12 skeletal myocytes via the activation of cAMP/PKA/CREB/PGC-1alpha signaling axis  [Not invited]
    第94回日本生理学会大会  2017
  • 循環生理学教育における個人的悩み：医学生理学でどこまで教えると良いのか？  [Not invited]
    第94回日本生理学会大会シンポジウム「生理学から薬理学への医学教育キャリーオーバー：何をどこまで教えればよいのか？」  2017
  • Involvement of Rho-Rho kinase pathway in peripheral actin filament formation, an initial event during thrombin-induced endothelial barrier disruption  [Not invited]
    第94回日本生理学会大会  2017
  • Mifepristone, a steroid receptor antagonist, induces hepatic steatosis in association with upregulation of resistin expression in liver  [Not invited]
    第94回日本生理学会大会  2017
  • Coagulation factor XIa induces Ca2+ influx via L-type Ca2+ channel in vascular smooth muscle cells  [Not invited]
    第94回日本生理学会大会  2017
  • NAD(P)H and low ionic strength suppresses dissociation of apoptosis-inducing factor from the E. coli membrane  [Not invited]
    第94回日本生理学会大会  2017
  • Proteinase-activated receptor-1はポドサイト障害によるアルブミン尿の病態に関与する  [Not invited]
    第１７回日本NO学会学術集会  2017
  • 血管内皮細胞のはたらき～バリアー機能について～  [Not invited]
    第5回香川県高校生科学研究発表会  2017
  • 血管平滑筋細胞において凝固第XI因子が引き起こすプロテイナーゼ活性化型受容体１を介した電位作動性カルシウムチャネル依存性Ca2+流入作用  [Not invited]
    第５９回日本平滑筋学会総会  2017
  • 実験的肺高血圧症に対するプロテイナーゼ活性化型受容体1（PAR1）拮抗薬の治療的効果  [Not invited]
    第５９回日本平滑筋学会総会  2017
  • 凝固第Ⅺ因子が引き起こす血管平滑筋細胞内Ca2+シグナルの発生メカニズム  [Not invited]
    生理研研究会2017心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明  2017
  • 血液凝固第XI因子が引き起こす血管平滑筋細胞のCa2+シグナルの発生メカニズムの解明  [Not invited]
    第69回日本生理学会中国四国地方会  2017
  • 嫌気条件とNAD(P)HはApoptosis-inducing factorの大腸菌膜からの解離を抑制する  [Not invited]
    第69回日本生理学会中国四国地方会  2017
  • トロンビン受容体PAR1の阻害は肺高血圧症の病態形成を抑制する  [Not invited]
    第132回日本薬理学会近畿部会  2017
  • 凝固第Ⅺ因子の血管平滑筋作用  [Not invited]
    第27回日本循環薬理学会  2017
  • Involvement of Rho-Rho kinase pathway in peripheral actin filament formation, an initial event during thrombin-induced endothelial barrier disruption  [Not invited]
    第94回日本生理学会大会  2017
  • 血管平滑筋細胞において凝固第XI因子が引き起こすプロテイナーゼ活性化型受容体１を介した電位作動性カルシウムチャネル依存性Ca2+流入作用  [Not invited]
    第５９回日本平滑筋学会総会  2017
  • 実験的肺高血圧症に対するプロテイナーゼ活性化型受容体1（PAR1）拮抗薬の治療的効果  [Not invited]
    第５９回日本平滑筋学会総会  2017
  • 凝固第Ⅺ因子が引き起こす血管平滑筋細胞内Ca2+シグナルの発生メカニズム  [Not invited]
    生理研研究会2017心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明  2017
  • 血液凝固第XI因子が引き起こす血管平滑筋細胞のCa2+シグナルの発生メカニズムの解明  [Not invited]
    第69回日本生理学会中国四国地方会  2017
  • 嫌気条件とNAD(P)HはApoptosis-inducing factorの大腸菌膜からの解離を抑制する  [Not invited]
    第69回日本生理学会中国四国地方会  2017
  • トロンビン受容体PAR1の阻害は肺高血圧症の病態形成を抑制する  [Not invited]
    第132回日本薬理学会近畿部会  2017
  • 凝固第Ⅺ因子の血管平滑筋作用  [Not invited]
    第27回日本循環薬理学会  2017
  • Increased contractile effects of thrombin in pulmonary artery of monocrotaline-induced pulmonary hypertension  [Not invited]
    第９３回日本生理学会大会  2016
  • Vascular intrinsic circadian rhythm of myosin phosphatase activity  [Not invited]
    第93回日本生理学会大会  2016
  • Increased expression of thrombin receptor, PAR1 is a new therapeutic target for pulmonary hypertension  [Not invited]
    第８０回日本循環器学会学術集会  2016
  • Mifepristone induces non-obese non-alcoholic fatty liver disease in association with upregulation of MGAT-1 expression  [Not invited]
    第８９回日本薬理学会年会  2016
  • Cell aging Is related to impairment of store-operated Ca2+ entry and endotheium-derived relaxing factor production in porcine aortic endothelial cells  [Not invited]
    The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide, The 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan  2016
  • Involvement of different subtypes of receptor in prostaglandin E2-induced motile function in lower esophageal sphincter and esophageal body smooth muscle  [Not invited]
    Digestive Disease Week 2016  2016
  • A key role of a second messenger cAMP in the induction of a transcriptional coactivator PGC-1α by a novel proangiogenic adenosine analogue COA-Cl in human fibroblasts  [Not invited]
    The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide, The 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan  2016
  • Trypsin induced a transient contraction via a PAR2/TRPV1/Neurokinin receptors pathway in circular smooth muscle of porcine esophageal body  [Not invited]
    Digestive Disease Week 2016  2016
  • モノクロタリン誘発肺高血圧症におけるトロンビン受容体PAR1の発現亢進とトロンビンの肺動脈収縮作用の増強  [Not invited]
    第１２９回日本薬理学会近畿部会  2016
  • モノクロタリン誘発肺高血圧モデルにおけるトロンビン受容体PAR1の肺動脈収縮作用の亢進  [Not invited]
    第５８回日本平滑筋学会総会  2016
  • Critical role of proteinase-activated receptor 1 in pathogenesis of pulmonary hypertension  [Not invited]
    6th Kagawa University-Chiang Mai University Joint Symposium  2016
  • 肺高血圧症におけるトロンビン受容体の役割  [Not invited]
    生理研研究会2016心臓・血管系の包括的な機能統合研究  2016
  • Specific and increased expression of thrombin receptor PAR1 in pulmonary artery plays a key role in the pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    American Heart Association Scientific Session 2016  2016
  • プロテイナーゼ活性化型受容体の血管病態生理学 ～脳血管攣縮と肺高血圧症を中心に～  [Not invited]
    第3回Heart & Brain Conference  2016
  • Coagulation factor XIa induces Ca2+ response in rat aorta smooth muscle cells via proteinase-activated receptor 1  [Not invited]
    第68回日本生理学会中国四国地方会  2016
  • 肺高血圧症の病態形成におけるトロンビン受容体PAR1の役割  [Not invited]
    第20回日本心血管内分泌代謝学会学術集会  2016
  • Peripheral actin bundle formation due to myosin light chain di-phosphorylation proceeds stress fiber formation during thrombin-induced endothelial barrier disruption  [Not invited]
    第33回国際心臓研究学会日本部会  2016
  • Vascular intrinsic circadian rhythm of myosin phosphatase activity  [Not invited]
    第93回日本生理学会大会  2016
  • Mifepristone induces non-obese non-alcoholic fatty liver disease in association with upregulation of MGAT-1 expression  [Not invited]
    第８９回日本薬理学会年会  2016
  • Cell aging Is related to impairment of store-operated Ca2+ entry and endotheium-derived relaxing factor production in porcine aortic endothelial cells  [Not invited]
    The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide, The 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan  2016
  • Involvement of different subtypes of receptor in prostaglandin E2-induced motile function in lower esophageal sphincter and esophageal body smooth muscle  [Not invited]
    Digestive Disease Week 2016  2016
  • A key role of a second messenger cAMP in the induction of a transcriptional coactivator PGC-1α by a novel proangiogenic adenosine analogue COA-Cl in human fibroblasts  [Not invited]
    The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide, The 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan  2016
  • Trypsin induced a transient contraction via a PAR2/TRPV1/Neurokinin receptors pathway in circular smooth muscle of porcine esophageal body  [Not invited]
    Digestive Disease Week 2016  2016
  • Critical role of proteinase-activated receptor 1 in pathogenesis of pulmonary hypertension  [Not invited]
    6th Kagawa University-Chiang Mai University Joint Symposium  2016
  • Specific and increased expression of thrombin receptor PAR1 in pulmonary artery plays a key role in the pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    American Heart Association Scientific Session 2016  2016
  • 小胞体Ca2+センサー蛋白質STIM1のリン酸化による貯蔵部作動性Ca2+流入の制御  [Not invited]
    第４４回日本心脈管作動物質学会  2015
  • DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis and cell morphology  [Not invited]
    第４４回日本心脈管作動物質学会  2015
  • The protective role of bone morphogenetic protein receptor II against thrombin-induced endothelial barrier dysfunction  [Not invited]
    第４４回日本心脈管作動物質学会  2015
  • 細胞辺縁部に局在するミオシン軽鎖２リン酸化とアクチン繊維束形成が内皮細胞バリアー障害の初期事象として重要な役割を果たす  [Not invited]
    第４４回日本心脈管作動物質学会  2015
  • トロンビン受容体の機能亢進がモノクロタリン誘発肺高血圧症の病態形成に重要な役割を果たす  [Not invited]
    第４４回日本心脈管作動物質学会  2015
  • A novel angiogenic agent COA-Cl induced PGC-1α-mediated VEGF production in cultured human fibroblasts.  [Not invited]
    第92回日本生理学会大会  2015
  • COA-Cl-induced adipogenesis is associated with an increased cell cycle progression and down-regulation of p27Kip1 in mouse pre-adipocyte 3T3-L1 cells  [Not invited]
    第92回日本生理学会大会  2015
  • Regulation of store-operated Ca2+ influx by STIM1 phosphorylation  [Not invited]
    第92回日本生理学会大会  2015
  • Increased thrombin receptor activity in monocrotaline-induced pulmonary hypertension.  [Not invited]
    第88回日本薬理学会年会  2015
  • A novel adenosine-like angiogenic agent COA-Cl elevates VEGF production via the transcription co-activator PGC-1α in cultured human fibroblasts  [Not invited]
    第79回日本循環器学会学術集会  2015
  • Potential of increased activity of proteinase-activated receptor 1 (PAR1) in pulmonary hypertension as a new therapeutic target.  [Not invited]
    第79回日本循環器学会学術集会  2015
  • Increased activity of proteinase activated receptor 1 (PAR1) plays a key role in pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    第79回日本循環器学会学術集会  2015
  • Proteinase activated receptor 1 (PAR1) plays a key role in pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Endogenous H2S contributes to myogenic tone generation in lower esophageal sphincter: possible involvement of Na+/Ca2+ exchanger  [Not invited]
    Digestive Disease Week 2015  2015
  • DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis and cell morphology  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • A role of myosin light chain phosphorylation and actin bundle formation at cell periphery as an initial event during thrombin-induced disruption of endothelial barrier  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Mechanism for impaired desensitization and irreversible activation of thrombin receptor in vascular smooth muscle  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Possible role of thrombin receptor in pathogenesis of pulmonary hypertension  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Store-operated phosphorylation of stromal interaction molecule 1 (STIM1) supports sustained Ca2+ entry  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Functional role of store-operated STIM1 phosphorylation  [Not invited]
    NIPS International Workshop ~ TRPs and SOCs Unconventional Ca2+ Physiology ~  2015
  • モノクロタリン誘発肺高血圧モデルにおける肺動脈のトロンビンに対する収縮反応性の亢進  [Not invited]
    第57回日本平滑筋学会  2015
  • ミオシン軽鎖リン酸化研究の新展開：循環器研究からシステム生物学へ  [Not invited]
    第57回日本平滑筋学会 シンポジウム「ミオシン軽鎖リン酸化研究の新展開：循環器研究からシステム生物学へ」  2015
  • ブタ下部食道括約筋の筋原性筋緊張調節における内因性硫化水素（H2S）の役割  [Not invited]
    第57回日本平滑筋学会  2015
  • 新規アデノシン類似化合物COA-Cl（コアクロル）はcAMP/CREB/PGC-1αの経路を介して血管内皮成長因子（VEGF）の産生を促進する  [Not invited]
    第67回日本生理学会中国四国地方会  2015
  • 新規アデノシン類似化合物COA-Cl（コアクロル）はヒト皮膚由来線維芽細胞において結合組織成長因子（CTGF）の発現を抑制する  [Not invited]
    第67回日本生理学会中国四国地方会  2015
  • トロンビン受容体PAR1拮抗薬は肺高血圧の病態形成を抑制する  [Not invited]
    第3回肺高血圧学会・第4回肺循環学会合同学術集会  2015
  • 内皮バリアー障害の初期事象としてのミオシン2リン酸化の特異的役割  [Not invited]
    生理研研究会2015心臓・血管系の包括的な機能統合研究  2015
  • トロンビンによる内皮バリアー機能障害の初期事象としてのミオシン軽鎖２リン酸化の特異的役割  [Not invited]
    第67回日本生理学会中国四国地方会  2015
  • 肺高血圧病態におけるトロンビン誘発大動脈収縮反応の増強  [Not invited]
    第６７回日本薬理学会西南部会  2015
  • 新規血管新生促進物質コアクロル（COA-Cl）による血管内皮成長因子（VEGF）産生機序の検討  [Not invited]
    第３回中国・四国地区医学生学術交流会  2015
  • A key role of PGC-1α co-transcription factor, but not HIF1α transcription factor, in production of vascular endothelial growth factor (VEGF) by a novel angiogenic agent COA-Cl in cultured human fibroblasts  [Not invited]
    American Heart Association Scientific Session 2015  2015
  • Activation of cAMP/CREB/PGC-1α pathway plays a key role for a novel adenosine-like angiogenic agent COA-Cl to induce production of vascular endothelial growth factor (VEGF) in cultured human fibroblasts  [Not invited]
    American Heart Association Scientific Session 2015  2015
  • Proteinase-activated receptor 1 antagonist inhibited the progression of monocrotaline induced pulmonary hypertension in rats  [Not invited]
    American Heart Association Scientific Session 2015  2015
  • COA-Cl-induced adipogenesis is associated with an increased cell cycle progression and down-regulation of p27Kip1 in mouse pre-adipocyte 3T3-L1 cells  [Not invited]
    第92回日本生理学会大会  2015
  • Regulation of store-operated Ca2+ influx by STIM1 phosphorylation  [Not invited]
    第92回日本生理学会大会  2015
  • Increased thrombin receptor activity in monocrotaline-induced pulmonary hypertension.  [Not invited]
    第88回日本薬理学会年会  2015
  • Potential of increased activity of proteinase-activated receptor 1 (PAR1) in pulmonary hypertension as a new therapeutic target.  [Not invited]
    第79回日本循環器学会学術集会  2015
  • Increased activity of proteinase activated receptor 1 (PAR1) plays a key role in pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    第79回日本循環器学会学術集会  2015
  • Proteinase activated receptor 1 (PAR1) plays a key role in pathogenesis of monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis and cell morphology  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • A role of myosin light chain phosphorylation and actin bundle formation at cell periphery as an initial event during thrombin-induced disruption of endothelial barrier  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Mechanism for impaired desensitization and irreversible activation of thrombin receptor in vascular smooth muscle  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Possible role of thrombin receptor in pathogenesis of pulmonary hypertension  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Store-operated phosphorylation of stromal interaction molecule 1 (STIM1) supports sustained Ca2+ entry  [Not invited]
    HAKATA Cardiovascular Conference  2015
  • Functional role of store-operated STIM1 phosphorylation  [Not invited]
    NIPS International Workshop ~ TRPs and SOCs Unconventional Ca2+ Physiology ~  2015
  • Apoptosis-inducing factor依存的細胞死の制御機構の解析  [Not invited]
    第55回（平成26年度）日本生化学会 中国・四国支部例会  2014
  • トロンビン受容体拮抗薬はモノクロタリンによる肺高血圧症の誘発を抑制する  [Not invited]
    第35回日本循環制御医学会総会  2014
  • ブタ食道体部縦走筋と輪走筋におけるトリプシンの作用とその機序  [Not invited]
    第56回日本平滑筋学会  2014
  • STIM1のリン酸化による貯蔵部作動性カルシウム流入の制御  [Not invited]
    第56回日本平滑筋学会  2014
  • 内皮バリアー障害における細胞辺縁部のミオシン軽鎖２リン酸化の役割  [Not invited]
    生理研研究会2014 心血管膜輸送分子の構造・機能・病態の統合的研究戦略  2014
  • Submembranous di-phosphorylation of myosin light chain and actin fiber formation play a critical role as an initial event during endothelial barrier disruption  [Not invited]
    11th International Symposium on Resistant Artery  2014
  • Vascular intrinsic biological clock in the regulation of myofilament calcium sensitivity.  [Not invited]
    11th International Symposium on Resistant Artery  2014
  • アデノシン類似化合物COA-ClによるVEGF分泌促進機序の検討  [Not invited]
    第66回日本生理学会中国四国地方会  2014
  • Inhibition of increased thrombin receptor activity attenuated monocrotaline-induced pulmonary hypertension in rats  [Not invited]
    American Heart Association Scientific Session 2014  2014
  • 大腸菌発現系を用いたApoptosis-inducing factor (AIF)と生体膜との相互作用の解析  [Not invited]
    日本生体エネルギー研究会 第４０回討論会  2014
  • Submembranous di-phosphorylation of myosin light chain and actin fiber formation play a critical role as an initial event during endothelial barrier disruption  [Not invited]
    11th International Symposium on Resistant Artery  2014
  • Vascular intrinsic biological clock in the regulation of myofilament calcium sensitivity.  [Not invited]
    11th International Symposium on Resistant Artery  2014

Works

  • 日本平滑筋学会ロゴマークデザイン
    Others 2014/08 - Today

MISC

  • 新規アデノシンアナログCOA-CIのインシュリン分泌促進作用
    塚本 郁子; Hossain Akram; 高田 麻紀; Liu Wenhua; 平野 勝也; 窪田 泰夫; 五十嵐 淳介; 小西 良士  日本薬学会年会要旨集  140年会-  27P  -am014  2020/03
  • N-terminal region of apoptosis-inducing factor facilitates dimer formation by stabilizing the charge transfer complex
    Tetsuo Yamashita; Takeshi Hashimoto; Junsuke Igarashi; Hiroaki Kosaka; Katsuya Hirano  The Journal of Physiological Science  70-  (Supplement 1)  S137  -S137  2020/03  [Refereed]
  • 血液凝固第XI因子の血管機能調節因子としての新たな機能の発見
    劉文華; 橋本剛; 山下哲生; 平野勝也  日本生理学雑誌(Web)  81-  (2)  2019
  • 血管平滑筋細胞において凝固第XI因子はトロンビン受容体PAR1を活性化し,細胞内Ca2+シグナルを発生させ,細胞遊走を促進する
    平野勝也; 劉文華; 橋本剛; 山下哲生  血管  42-  (1)  2019
  • 競合および混合阻害剤との共結晶構造解析により明らかとなった酵母由来Type II NADH脱水素酵素のユビキノン結合部位
    山下哲生; 稲岡健ダニエル; 志波智生; 三芳秀人; 原田繁春; 北潔; 平野勝也  日本農芸化学会中四国支部講演会講演要旨集(Web)  51st-  2018
  • 血液凝固第XI因子が引き起こす血管平滑筋細胞のCa2+シグナルの発生メカニズムの解明
    劉文華; 橋本剛; 山下哲生; 平野勝也  日本生理学雑誌(Web)  80-  (3)  2018
  • 嫌気条件とNAD(P)HはApoptosis-inducing factorの大腸菌膜からの解離を抑制する
    山下哲生; 松本感; 橋本剛; 五十嵐淳介; 小坂博昭; 平野勝也  日本生理学雑誌(Web)  80-  (3)  2018
  • 血液凝固第XI因子は電位作動性Ca2+チャネルを介して血管平滑筋細胞にCa2+シグナルを発生させる
    平野勝也; 劉文華; 橋本剛; 山下哲生  血管  41-  (1)  2018
  • ポドサイト障害進展におけるProteinase‐activated receptor‐1の関与
    中野大介; GUAN Yu; 人見浩史; 平野勝也; 桑原孝成; 森潔; 向山政志; 西山成  日本腎臓学会誌  59-  (6)  703  -703  2017/09
  • Proteinase‐activated receptor‐1はポドサイト障害によるアルブミン尿の病態に関与する
    管有; 中野大介; 人見浩史; 平野勝也; 西山成  日本NO学会学術集会プログラム抄録集  17th-  57  2017/05
  • Apoptosis-inducing factorと大腸菌膜との相互作用の解析
    松本感; 山下哲生; 橋本剛; 五十嵐淳介; 小坂博昭; 平野勝也  日本生理学雑誌(Web)  79-  (1)  2017
  • 凝固第XI因子が引き起こす血管平滑筋細胞のカルシウムシグナル伝達
    平野勝也; 劉文華; 橋本剛; 山下哲生; 五十嵐淳介  血管  40-  (1)  2017
  • 凝固第XI因子の血管平滑筋作用
    平野勝也; 劉文華; 橋本剛; 山下哲生  日本循環薬理学会口演要旨集  27th-  2017
  • NAKANO Daisuke; GUAN Yu; LIU Wenhua; ZHANG Yifan; LI Lei; HITOMI Hirofumi; HIRANO Katsuya; NISHIYAMA Akira  Annual Meeting of the Japanese Society of Toxicology  44-  (0)  O  -53  2017
  • Endogenous H2S maintains myogenic tone in lower esophageal sphincter: A possible target for treatment of lower esophageal sphincter dysfunction
    Xiaopeng Bai; Eikichi Ihara; Yoshimasa Tanaka; Katsuya Hirano; Mayumi Hirano; Kazuhiko Nakamura  JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY  31-  11  -11  2016/11
  • Trypsin Induced a Transient Contraction via a PAR2/TRPV1/Neurokinin Receptors Pathway in Circular Smooth Muscle of Porcine Esophageal Body
    Xiaopeng Bai; Eikichi Ihara; Yoshimasa Tanaka; Katsuya Hirano; Mayumi Hirano; Hirotada Akiho; Kazuhiko Nakamura  GASTROENTEROLOGY  150-  (4)  S458  -S458  2016/04
  • Involvement of Different Subtypes of Receptor in Prostaglandin E2-Induced Motile Function in Lower Esophageal Sphincter and Esophageal Body Smooth Muscle
    Xiaopeng Bai; Eikichi Ihara; Yoshimasa Tanaka; Katsuya Hirano; Mayumi Hirano; Kazuhiko Nakamura  GASTROENTEROLOGY  150-  (4)  S458  -S458  2016/04
  • 新規アデノシン類似化合物COA-Cl(コアクロル)はcAMP/CREB/PGC-1αの経路を介して血管内皮成長因子(VEGF)の産生を促進する
    岡本隆司; 五十嵐淳介; 橋本剛; 山下哲生; 苅田咲子; 窪田泰夫; 高田麻紀; 小西良士; 塚本郁子; 平野勝也  日本生理学雑誌(Web)  78-  (1)  2016
  • Activation of cAMP/CREB/PGC-1 alpha Pathway Plays a Key Role for a Novel Adenosine-like Angiogenic Agent COA-CI to Induce Production of Vascular Endothelial Growth Factor (VEGF) in Cultured Human Fibroblasts
    Ryuji Okamoto; Junsuke Igarashi; Takeshi Hashimoto; Tetsuo Yamashita; Kazuyo Shoji; Yasuo Kubota; Norikazu Sakakibara; Ikuko Tsukamoto; Ryoji Konishi; Katsuya Hirano  CIRCULATION  132-  2015/11
  • Proteinase-activated Receptor 1 Antagonist Inhibited the Progression of Monocrotaline Induced Pulmonary Hypertension in Rats
    Yukimitsu Kuwabara; Kohtaro Abe; Mayumi Hirano; Yoshitaka Hirooka; Kenji Sunagawa; Katsuya Hirano  CIRCULATION  132-  2015/11
  • A Key Role of PGC-1 alpha Co-transcription Factor, but not HIF1 alpha Transcription Factor, in Production of Vascular Endothelial Growth Factor (VEGF) by a Novel Angiogenic Agent COA-CI in Cultured Human Fibroblasts
    Ryuji Okamoto; Junsuke Igarashi; Tetsuo Yamashita; Takeshi Hashimoto; Sakiko Karita; Kazuyo Shoji; Yasuo Kubota; Maki Takata; Fuminori Yamaguchi; Masaaki Tokuda; Norikazu Sakakibara; Ikuko Tsukamoto; Ryoji Konishi; Katsuya Hirano  CIRCULATION  132-  2015/11
  • Endogenous H2S Contributes to Myogenic Tone Generation in Lower Esophageal Sphincter: Possible Involvement of Na+/CA(2+) Exchanger
    Xiaopeng Bai; Eikichi Ihara; Yoshimasa Tanaka; Katsuya Hirano; Mayumi Hirano; Hirotada Akiho; Kazuhiko Nakamura; Ryoichi Takayanagi  GASTROENTEROLOGY  148-  (4)  S78  -S78  2015/04
  • DHRS7C NAD/NADH dehydrogenase catalytic core domain is essential for cellular calcium homeostasis and cell morphology
    新井しのぶ; 井手友美; 池田昌隆; 平野勝也; 松尾祐佳; 砂川賢二  血管  38-  (1)  2015
  • Inhibition of Increased Thrombin Receptor Activity Attenuated Monocrotaline-induced Pulmonary Hypertension in Rats
    Yukimitsu Kuwabara; Kohtaro Abe; Mayumi Hirano; Yoshitaka Hirooka; Katsuya Hirano; Kenji Sunagawa  CIRCULATION  130-  2014/11
  • Different Contractile and Relaxant Effects of Trypsin in Phasic Smooth Muscles of the Esophageal Body and the Tonic Lower Esophageal Sphincter
    Xiaopeng Bai; Yoshimasa Tanaka; Eikichi Ihara; Katsuya Hirano; Mayumi Hirano; Kazuhiko Nakamura; Hirotada Akiho; Ryoichi Takayanagi  GASTROENTEROLOGY  146-  (5)  S862  -S862  2014/05
  • 3T3-L1細胞の脂肪細胞分化に及ぼすミフェプリストンの促進作用
    橋本剛; 五十嵐淳介; 山下哲生; 平野勝也  肥満研究  20-  (Supplement)  2014
  • 大腸菌発現系を用いたApoptosis-inducing factor(AIF)と生体膜との相互作用の解析
    山下哲生; 橋本剛; 五十嵐淳介; 平野勝也; 小坂博昭  日本生体エネルギー研究会討論会講演要旨集  40th-  2014
  • Vascular intrinsic biological clock in the regulation of myofilament calcium sensitivity
    Katsuya Hirano  JOURNAL OF VASCULAR RESEARCH  51-  65  -65  2014
  • Submembranous di-phosphorylation of myosin light chain and actin fiber formation play a critical role as an initial event during endothelial barrier disruption
    Mayumi Hirano; Katsuya Hirano  JOURNAL OF VASCULAR RESEARCH  51-  106  -106  2014
  • Mechanisms underlying impaired desensitization of proteinase-activated receptor 1 (PAR(1))
    Katsuya Hirano; Mayumi Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  124-  160P  -160P  2014
  • Role of mono- and di-phosphorylation of myosin light chain in thrombin-induced endothelial barrier disruption
    Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  124-  223P  -223P  2014
  • Trypsin Induced BiPhasic Contraction and Relaxation in the Porcine Lower Esophageal Sphincter
    Yoshimasa Tanaka; Eikichi Ihara; Katsuya Hirano; Mayumi Hirano; Kazuhiko Nakamura; Hirotada Akiho; Ryoichi Takayanagi  GASTROENTEROLOGY  144-  (5)  S857  -S857  2013/05
  • Rho Kinase-mediated Di-phosphorylation of Myosin Light Chain in the Sub-membranous Regions and Circumferential Actomyosin Contraction Mediate Thrombin-induced Barrier Disruption in Vascular Endothelial Cells
    Mayumi Hirano; Katsuya Hirano  STROKE  44-  (2)  2013/02
  • Oxidative stress and Extracellular Signal-regulated Kinase (ERK) Play a Key Role in Impairment of Receptor Desensitization and Prolongation of Vascular Reactivity in the Cerebral Artery after Subarachnoid Hemorrhage
    Katsuya Hirano; Katsuharu Kameda; Akiko Hanada; Mayumi Hirano  STROKE  44-  (2)  2013/02
  • Trypsin induced biphasic contraction and relaxation in the porcine lower esophageal sphincter
    Yoshimasa Tanaka; Eikichi Ihara; Katsuya Hirano; Mayumi Hirano; Kazuhiko Nakamura; Ryoichi Takayanagi  JOURNAL OF PHARMACOLOGICAL SCIENCES  121-  169P  -169P  2013
  • A Clock Gene ROR alpha-mediated Regulation of the Activity of Rho-associated Coiled-coil Protein Kinase 2 (ROCK2) Plays a Key Role in Generating Vascular Intrinsic Circadian Rhythm of Myofilament Ca2+ Sensitivity and Vascular Contractility
    Toshiro Saito; Mayumi Hirano; Tomomi Ide; Toshihiro Ichiki; Noriyuki Koibuchi; Kenji Sunagawa; Katsuya Hirano  CIRCULATION RESEARCH  111-  (12)  E382  -E382  2012/12
  • Oxidative Stress and Rho-Associated Coiled-Coil Protein Kinase (ROCK)-Mediated Double Phosphorylation of Myosin Light Chain in the Submembranous Region Plays a Key Role in Thrombin-Induced Barrier Dysfunction in Vascular Endothelial Cells
    Mayumi Hirano; Akiko Hanada; Katsuya Hirano  CIRCULATION  126-  (21)  2012/11
  • Oxidative Stress Impairs Desensitization of G-protein Coupled Receptors and Increases Vascular Reactivity in Basilar Artery after Subarachnoid Hemorrhage (SAH)
    Katsuharu Kameda; Yuichiro Kikkawa; Mayumi Hirano; Satoshi Matsuo; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  118-  78P  -78P  2012
  • Rho kinase-mediated di-phosphorylation of myosin light chain in the subjunctional regions plays a critical role in thrombin-induced endothelial barrier dysfunction
    Mayumi Hirano; Akiko Hanada; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  118-  245P  -245P  2012
  • Enhancement of Endothelin-1-induced myofilament sensitization to Ca2+ and its mechanisms after subarachnoid hemorrhage (SAH)
    Yuichiro Kikkawa; Satoshi Matsuo; Mayumi Hirano; Akira Nakamizo; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  118-  83P  -83P  2012
  • Thrombin receptor antagonists
    HIRANO Katsuya  日本薬理學雜誌 = Folia pharmacologica Japonica  138-  (6)  258  -259  2011/12
  • Oxidative Stress Impairs Desensitization of the G-Protein Coupled Receptors (GPCRs) and Increases Vascular Reactivity by Prolonging the Contractile Responses in the Cerebral Arteries After Subarachnoid Hemorrhage
    Katsuharu Kameda; Yuichiro Kikkawa; Mayumi Hirano; Satoshi Matsuo; Tomio Sasaki; Katsuya Hirano  CIRCULATION  124-  (21)  2011/11
  • Ca2+-Independent Di-phosphorylation of Myosin Light Chain by Rho-associated Coiled-Coil Protein Kinase (ROCK) in the Subjunctional Regions of Cell-Cell Contact Mediates Thrombin-Induced Barrier Dysfunction in Vascular Endothelial Cells
    Mayumi Hirano; Akiko Hanada; Katsuya Hirano  CIRCULATION  124-  (21)  2011/11
  • Depletion of Intracellular Ca2+ Stores Induces Phosphorylation of Stromal Interaction Molecular 1 (STIM1), Which Contributes to the Sustained Phase of Store-operated Ca2+ Influx in Vascular Endothelial Cells
    Katsuya Hirano; Akiko Hanada; Mayumi Hirano  CIRCULATION  124-  (21)  2011/11
  • くも膜下出血後の血管反応性亢進に、受容体活性および筋フィラメントのカルシウム感受性に対するフィードバック調節機構の障害が関与する
    吉川 雄一郎; 平野 勝也; 亀田 勝治; 中溝 玲; 吉本 幸司; 溝口 昌弘; 佐々木 富男  脳血管攣縮  26-  73  -78  2011/02
  • ROCK2 plays a key role in the circadian change in thrombin-induced myosin light chain phosphorylation in smooth muscle
    Toshiro Saito; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  115-  160P  -160P  2011
  • The combination of argatroban and vitamin C normalizes the increased vascular reactivity after subarachnoid hemorrhage (SAH)
    Katsuharu Kameda; Yuichiro Kikkawa; Mayumi Hirano; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  115-  72P  -72P  2011
  • Store-operated phosphorylation of endogenous STIM1 in the interphase cells as revealed by a Phos-tag SDS-PAGE analysis
    Katsuya Hirano; Akiko Hanada; Mayumi Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  115-  272P  -272P  2011
  • Thrombin causes barrier dysfunction via two distinct signal transduction pathways in vascular endothelial cells
    Mayumi Hirano; Akiko Hanada; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  115-  157P  -157P  2011
  • Identification of Rho-associated Kinase 2 (ROCK2) as a Key Molecule Generating the Intrinsic Circadian Rhythm of Vascular Contractility
    Toshiro Saito; Mayumi Hirano; Tomomi Ide; Kenji Sunagawa; Katsuya Hirano  CIRCULATION  122-  (21)  2010/11
  • Rho-associated Kinase (ROCK) Mediates the Thrombin-Induced Production of Reactive Oxygen Species and Myosin Light Chain Phosphorylation-Independent Vasoconstriction in the Pulmonary Artery
    Katsuya Hirano; Jun Maki; Mayumi Hirano  CIRCULATION  122-  (21)  2010/11
  • Intrinsic circadian oscillation of myosin light chain phosphorylation in vascular smooth muscle cells
    Toshiro Saito; Mayumi Hirano; Tomomi Ide; Kenji Sunagawa; Katsuya Hirano  FASEB JOURNAL  24-  2010/04
  • くも膜下出血後のウサギ脳底動脈におけるG蛋白質共役型受容体の脱感作障害
    吉川 雄一郎; 平野 勝也; 亀田 勝治; 甲斐 康稔; 宮城 靖; 溝口 昌弘; 庄野 禎久; 佐々木 富男  脳血管攣縮  25-  23  -25  2010/03
  • Differential regulation of the thrombin-induced mono- and di-phosphorylation of myosin light chain in vascular endothelial cells
    Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  112-  144P  -144P  2010
  • The thrombin-induced myosin light chain phosphorylation exhibits an intrinsic circadian oscillation in vascular smooth muscle
    Toshiro Saito; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  112-  149P  -149P  2010
  • Mechanisms underlying increased vascular contractility in subarachnoid hemorrhage
    Yuichiro Kikkawa; Katsuharu Kameda; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHYSIOLOGICAL SCIENCES  60-  S57  -S57  2010
  • Impaired feedback regulation of the receptor signaling and the myofilament Ca2+ sensitization contributes to increased vascular reactivity after subarachnoid hemorrhage
    Yuichiro Kikkawa; Katsuharu Kameda; Mayumi Hirano; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  112-  60P  -60P  2010
  • The combination of thrombin inhibitor and vitamin C normalizes the increased vascular reactivity after subarachnoid hemorrhage
    Katsuharu Kameda; Yuichiro Kikkawa; Mayumi Hirano; Tomio Sasaki; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  112-  148P  -148P  2010
  • Regulation and physiological roles of store-operated Ca2+ influx in vascular endothelial cells
    Katsuya Hirano; Mayumi Hirano  JOURNAL OF PHYSIOLOGICAL SCIENCES  60-  S55  -S55  2010
  • Intracellular alkalinization activates STIM1-dependent but store-independent Ca2+ influx in vascular endothelial cells
    Katsuya Hirano; Akiko Hanada; Mayumi Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  112-  144P  -144P  2010
  • Thrombin Activation of Proteinase-Activated Receptor 1 (PAR(1)) Induces Ca2+-Independent Vasoconstriction by Activating the Production of Reactive Oxygen Species in the Pulmonary Artery
    Jun Maki; Mayumi Hirano; Katsuya Hirano  CIRCULATION  120-  (18)  S1022  -S1023  2009/11
  • Roles of proteinase-activated receptors in the increased detrusor muscle contractility and pollakisuria in cystitis in mice
    Ken-ichiro Shiga; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  144P  -144P  2009
  • A new method for analyzing myosin light chain phosphorylation with a Phos-tag (TM) technology
    Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  259P  -259P  2009
  • The impairment. of the desensitization of G protein-coupled receptors in subarachnoid hemorrhage in rabbit basilar arteries
    Yuichiro Kikkawa; Katsuharu Kameda; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  63P  -63P  2009
  • Involvement of STIM1 in PAR1-mediated Ca2+ influx and NO production in vascular endothelial cells
    Katsuya Hirano; Akiko Hanada; Mayumi Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  265P  -265P  2009
  • Identification of a novel STIM1-Binding protein as a regulator of store-operated Ca2+ influx
    Katsuya Hirano; Akiko Hanada; Mayumi Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  55P  -55P  2009
  • Preventive effect of thrombin inhibitor argatroban on increased contractile response to thrombin in the basilar artery after subarachnoid hemorrhage
    Katsuharu Kameda; Yuichiro Kikkawa; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  231P  -231P  2009
  • Roles of reactive oxygen species (ROS) and Rho-kinase in the thrombin-induced and proteinase-activated receptor 1(PAR(1))-mediated pulmonary artery vasoconstriction
    Jun Maki; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  109-  259P  -259P  2009
  • PJ-539 Novel Role of Rac1 in the Transcriptional Up-regulation of p27^ and Cell Cycle Arrest in Vascular Endothelial Cells(Molecular biology, vascular(02)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Mayumi; Kanaide Hideo; Hirano Katsuya  Circulation journal : official journal of the Japanese Circulation Society  72-  648  -648  2008/03
  • PE-164 Up-regulation of Proteinase-Activated Receptor 2 by Oxidative Stress in Vascular Endothelial Cells both in vitro and in vivo(Cardiovascular pharmacology, basic/clinical(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  72-  401  -401  2008/03
  • OJ-066 Cyclooxygenase Plays a Key Role in the Up-regulation of PAR1 and the Hyper-contractile Response to Thrombin after Balloon Injury(Cardiovascular pharmacology, basic/clinical(02)(H),Oral Presentation(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  72-  304  -304  2008/03
  • OE-229 Thrombin Stimulation of Proteinase-Activated Receptor 1 (PAR1) Potentiated Ca2+-Independent and Rho kinase-Mediated Contractile Mechanism in the Normal Pulmonary Artery(Pulmonary circulation(01)(H),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  72-  237  -238  2008/03
  • Enhancement of the bradykinin-induced contraction by thrombin and trypsin in the detrusor muscle of cyclophosphamide-treated mice
    Ken-ichiro Shiga; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  106-  203P  -203P  2008
  • Proteinase-Activated Receptor 1 (PAR1) induces Ca2+-independent, Rho kinase-mediated contraction in the normal pulmonary artery
    Jun Maki; Mayumi Hirano; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  106-  69P  -69P  2008
  • The role of thrombin receptor in cerebral vasospasm after subarachnoid hemorrhage
    Yasutoshi Kai; Yoshihisa Maeda; Tomio Sasaki; Hideo Kanaide; Katsuya Hirano  JOURNAL OF PHARMACOLOGICAL SCIENCES  106-  23P  -23P  2008
  • Regulatory mechanism of contraction in the dedifferentiated vascular smooth muscle
    平野 勝也; 金出 英夫  医学のあゆみ  223-  (13)  1113  -1118  2007/12
  • PE-422 Distinct Ca^<2+> Requirement for Endothelial NO Synthase-mediated NO Production Between Proteinase-Activated Receptor-1 and 4(Endothelium-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  71-  430  -430  2007/03
  • OE-326 Thrombin Activation of Proteinase-Activated Receptor 1 (PAR1) Potentiates Myofilament Ca^<2+> Sensitivity and Induces Vasoconstriction in Pulmonary Artery(Pulmonary circulation-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  71-  232  -232  2007/03
  • Nipradilol decreases Ca2+ sensitivity of pig ciliary artery
    Yuhei Hasegawa; Katsuya Hirano; Junji Nishimura; Mayumi Hirano; Hideo Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  103-  270P  -270P  2007
  • Plasmin induces relaxation through the activation of a novel proteinase-activated receptor in the porcine coronary artery
    Tetsuhiro Fujisyohi; Katsuya Hirano; Mayumi Hirano; Hideo Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  103-  194P  -194P  2007
  • Involvement of cGMP-mediated activation of Na+-Ca2+ exchanger in isosorbide dinitrate-induced decreases in [Ca2+](i) in vascular smooth muscle
    Eiji Karashima; Jun Yamanaka; Katsuya Hirano; mayumi Hirano; Takahiro Iwamoto; Satomi Kita; Hideo Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  103-  204P  -204P  2007
  • Involvement of Na+-Ca2+ exchange in replenishing the Ca2+ stores in vascular smooth muscle
    Jun Yamanaka; Katsuya Hirano; Eiji Karashima; Mayurm Hirano; Hideo Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  103-  152P  -152P  2007
  • Oxidative stress enhanced a proteinase-activated receptor-2-mediated endothelium-dependent relaxation in the rat aorta
    Murasaki Aman; Katsuya Hirano; Mayumi Hirano; Hideo Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  103-  247P  -247P  2007
  • 血管平滑筋収縮--MLCK作用を中心に (特集・血管壁)
    平野 勝也; 金出 英夫  生体の科学  57-  (6)  554  -559  2006/11
  • A novel strategy to prevent the vasospastic response of the basilar artery to thrombin in subarachnoid hemorrhage using a selective proteinase-activated receptor-1 antagonist
    Yasutoshi Kai; Katsuya Hirano; Junji Nishimura; Hideo Kanaide  CIRCULATION  114-  (18)  158  -158  2006/10
  • W-1.子宮平滑筋におけるプロテイナーゼ活性化型受容体の性ホルモンによる発現調節(産婦人科領域における最近の平滑筋研究,ワークショップ,第48回 日本平滑筋学会総会)
    平野 勝也; 阿萬 紫; 金出 英夫  日本平滑筋学会雑誌  10-  (1)  "J  -19"  2006/04
  • PJ-647 Plasmin Induces Endothelium-dependent Nitric Oxide-mediated Relaxation in Coronary Artery(Cardiovascular pharmacology, basic/clinical-5 (H) PJ109,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
    Fujiyoshi Tetsuhiro; Kanaide Hideo; Nishimura Junji; Hirano Katsuya; Hirano Mayumi  Circulation journal : official journal of the Japanese Circulation Society  70-  649  -650  2006/03
  • PE-189 A Novel Thrombin Receptor Antagonist Prevented Vasospastic Response to Thrombin in the Basilar Artery after Subarachnoid Hemorrhage(Cerebrovascular circulation/Stroke-2 (IHD) PE32,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
    Kai Yasutoshi; Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  70-  382  -382  2006/03
  • PE-572 Proteinase-Activated Receptor-4 Preferentially Activates Ca^<2+>-independent, Akt-mediated NO Production in Vascular Endothelial Cells(Endothelium-4 (H) PE99,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  70-  475  -475  2006/03
  • Homophilic cell contact activated Rac1 and up-regulated p27(Kip1) transcription in vascular endothelial cells
    M Hirano; K Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  182P  -182P  2006
  • Relaxin relaxes rat myometrium by decreasing Ca2+ sensitivity of contractile apparatus via a cAMP/PKA signaling pathway
    M Miyazaki; N Niiro; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  62P  -62P  2006
  • Balloon injury up-regulates the expression of proteinase-activated receptors and induces vasospasm at the injury site in rabbit femoral artery
    R Fukunaga; K Hirano; M Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  61P  -61P  2006
  • Proteinase-activated receptor (PAR)-1 activation potentiates myofilament Ca2+ sensitivity and induces pulmonary artery contraction
    J Maki; K Hirano; M Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  278P  -278P  2006
  • Oxidative stress enhanced a trypsin-induced endothelium-dependent relaxation in the rat aorta
    M Aman; K Hirano; M Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  280P  -280P  2006
  • Plasmin-induced endothelium-dependent relaxation in coronary artery
    T Fujiyoshi; K Hirano; M Hirano; N Niiro; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  277P  -277P  2006
  • Mechanism underlying the dimethyl sulfoxide (DMSO)-induced relaxation of the rabbit detrusor smooth muscle
    K Shiga; J Nishimura; N Niiro; M Hirano; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  214P  -214P  2006
  • A crucial role of Rac1 in membrane targeting of thrombin receptor in vascular smooth muscle cells
    W Eto; K Hirano; M Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  280P  -280P  2006
  • Prevention of hyper-contractile response to thrombin by proteinase-activated receptor-1 antagonist in rabbit double subarachnoid hemorrhage model
    Y Kai; K Hirano; M Hirano; N Niiro; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  100-  106P  -106P  2006
  • The vascular injury up-regulates the expression of proteinase-activated receptors and induces vasospasm at the injured site
    R Fukunaga; K Hirano; M Hirano; J Nishimura; Y Maehara; H Kanaide  CIRCULATION  112-  (17)  U76  -U76  2005/10
  • ヨード造影剤による腎尿細管細胞障害に対するセラミド合成阻害薬の保護作用
    矢野 貴久; 伊藤 善規; 千堂 年昭; 窪田 敏夫; 末安 正典; 平野 勝也; 金出 英夫; 大石 了三  日本薬学会年会要旨集  125年会-  (3)  142  -142  2005/03
  • The Phosphatidylinositol 3-kinase/Akt Pathway Mediates NO-independent Anti-apoptotic Effect of Estrogen in Vascular Endothelial Cells(Apoptosis/Necrosis/Regeneration 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  69-  190  -190  2005/03
  • Vascular Injury Induces Hyper-contractility Mediated by Proteinase-Activated Receptors (Vascular Smooth Muscle 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
    Fukunaga Ryota; Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  69-  556  -556  2005/03
  • Plasmin Antagonizes the Thrombin-induced Endothelium-dependent Relaxation in Coronary Artery (Cardiovascular Pharmacology, Basic/Clinical 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
    Fujiyoshi Tetsuhiro; Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  69-  599  -599  2005/03
  • Preventive Effect of a Thrombin Receptor Antagonist on Vasospasm in Subarachnoid Hemorrhage(Cerebrovascular Circulation/Stroke 1 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
    Kai Yasutoshi; Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  69-  508  -509  2005/03
  • Involvement of Na^+-Ca^<2+> Exchanger in Forskolin-induced Vasorelaxation in Mouse Thoracic Aorta : Evaluation Using NCX Transgenic Mouse Model(Vascular Smooth Muscle 1 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
    Karashima Eiji; Nishimura Junji; Iwamoto Takahiro; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  69-  175  -175  2005/03
  • Activation of proteinase-activated receptor-4 induces Ca2+-independent NO production in cultured bovine aortic endothelial cells
    N Nomoto; M Hirano; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  110P  -110P  2005
  • RhoA plays a key role in regulating NO production in in situ enclothelial cells
    N Shiga; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  296P  -296P  2005
  • Forskolin-induced vasorelaxation involves Na+/Ca2+ exchanger in mouse aorta
    E Karashima; J Nishimura; T Iwamoto; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  244P  -244P  2005
  • The Ca2+ refilling to the sarcoplasmic reticulum is important for the agonist-induced tonic contraction in the porcine coronary artery
    R Fukunaga; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  116P  -116P  2005
  • Antagonizing effect of plasmin on the thrombin-induced endothelium-dependent relaxation in coronary artery
    T Fujiyoshi; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  296P  -296P  2005
  • Proteinase-activated receptor-1 antagonist prevented hyper-contractile response to thrombin in rabbit subarachnoid hemorrhage model
    Y Kai; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  98P  -98P  2005
  • Transduction of Rac1/Cdc42-inhibitory protein down-regulated proteinase-activated receptor-1 and attenuated responsiveness to thrombin in human coronary artery smooth muscle cells
    T Yufu; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  287P  -287P  2005
  • The membrane trafficking plays an important role in maintaining the surface expression of proteinase-activated receptor-1 in vascular smooth muscle cells
    W Eto; M Hirano; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  295P  -295P  2005
  • Substance P increases Ca2+ sensitivity of pig iris sphincter muscle (ISM)
    Y Hasegawa; J Nishimura; K Hirano; M Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  167P  -167P  2005
  • De novo ceramide synthesis is involved in nephrotoxicity induced by radiographic contrast medium
    T Yano; Y Itoh; T Sendo; T Kubota; K Hirano; H Kanaide; R Oishi  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  96P  -96P  2005
  • Thrombin and trypsin potentiate myofilament Ca2+ sensitivity and induce contraction in pulmonary artery
    J Mai; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  288P  -288P  2005
  • Involvement of Na+/Ca2+ exchanger (NCX) in vasodilator-induced vasorelaxation
    J Nishimura; J Yamanaka; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  97-  56P  -56P  2005
  • Long-term inhibition of RhoA enhances endothelial NO production and reduces vascular tone in an intact artery
    N Shiga; K Hirano; J Nishimura; H Kanaide  CIRCULATION  110-  (17)  154  -154  2004/10
  • Thrombin induces vascular hypercontractility by upregulating protease-activated receptor-1 in subarachnoid hemorrhage
    Y Maeda; K Hirano; M Hirano; J Nishimura; T Sasaki; H Kanaide  CIRCULATION  110-  (17)  332  -333  2004/10
  • Engagement of Na+-Ca2+ exchanger and Na+-K+ ATPase in the coronary artery relaxation induced by isosorbide dinitrate
    J Yamanaka; J Nishimura; K Hirano; H Kanaide  JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY  37-  (1)  232  -232  2004/07
  • FRS-080 17 β-estradiol inhibits TNF-α induced apoptosis by activating the phosphatidylinositol 3-kinase/Akt pathway in the vascular endothelial cells(NO and Cardiovascular System (H) : FRS10)(Featured Research Session (English))
    Hirano Mayumi; Hirano Katsuya; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  110  -111  2004/03
  • PJ-150 Identification of cleavage sites responsible for a novel proteolytic mechanism of inactivation of protease-activated receptor-1 in vascular endothelial cells(Endothelium/NO 2 (H) : PJ25)(Poster Session (Japanese))
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  516  -516  2004/03
  • PE-232 Contractile Property of the Proliferating Vascular Smooth Muscle Cells : A Crucial Role of RhoA in Contractility(Vascular Smooth Muscle 1 (H) : PE40)(Poster Session (English))
    Nishimura Junji; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  419  -419  2004/03
  • PJ-367 Identification of a novel transcriptional regulatory element of p27^ gene that responds to formation of cell-cell contact in endothelial cells(Molecular Biology, Vascular 2 (H) : PJ61)(Poster Session (Japanese))
    Hirano Mayumi; Hirano Katsuya; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  570  -570  2004/03
  • PJ-364 A role of Pad in maintenance of cell surface expression of protease-activated receptor-1 in the vascular smooth muscle cells(Molecular Biology, Vascular 2 (H) : PJ61)(Poster Session (Japanese))
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  569  -569  2004/03
  • OJ-422 Inhibition of RhoA signalling enhances NO production in endothelial cells and reduces contractility in the isolated rabbit mesenteric artery(Endothelium/NO 5 (H) : OJ51)(Oral Presentation (Japanese))
    Hirano Katsuya; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  68-  332  -332  2004/03
  • Inhibition of Rac1 downregulates protease-activated receptor (PAR) 1 in the vascular smooth muscle cells
    T Yufu; K Hirano; D Bi; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  156P  -156P  2004
  • A novel type of protease-activated receptor (PAR) mediates an enhanced myometrial response to trypsin in sex hormone-treated rats
    M Aman; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  182P  -182P  2004
  • TGF-beta 1 enhances the fibroblast-mediated collagen gel contraction but not the isometric tension deveolpment
    S Ise; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  247P  -247P  2004
  • 17 beta-estradiol inhibits TNF-alpha induced apoptosis via phosphatidylinositol 3-kinase/Akt pathway in vascular endothelial cells
    M Koga; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  152P  -152P  2004
  • Relative contribution of PKC and Rho kinase in the contraction induced by PDBu, high K+ and U46619
    E Karashima; J Nishimura; K Hirano; M Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  145P  -145P  2004
  • Prostaglandin F2 alpha (PGF2 alpha) increases Ca(2+) sensitivity of pig iris sphincter muscle
    Y Hasegawa; Y Hasegawa; J Nishimura; K Hirano; M Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  142P  -142P  2004
  • Stimulation of Na+-Ca2+ exchanger by isosorbide dinitrate through Na+-K+ ATPase activation
    J Yamanaka; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  213P  -213P  2004
  • Role of PKC in contraction of human prostatic stromal cells
    R Takahashi; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  263P  -263P  2004
  • Homophilic cell-cell contact up-regulated transcription of p27(Kip1) gene in endothelial cells
    M Hirano; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  128P  -128P  2004
  • RNA interference in vascular smooth muscle cells: A crucial role of rhoA in contractility
    D Bi; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  145P  -145P  2004
  • Regulatory mechanism for the expression of protease-activated receptors: A role of small G proteins
    K Hirano; T Yufu; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  64P  -64P  2004
  • Effect of hypergravity on the cell cycle progression in the vascular endothelial cells
    K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  133P  -133P  2004
  • Subarachnoid hemorrhage induced hypercontractile response to thrombin in the rabbit basilar artery
    Y Maeda; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  98P  -98P  2004
  • Mechanism of the contraction induced by bradykinin in the guinea-pig taenia coli
    H Kawata; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  94-  77P  -77P  2004
  • A fundamental fole of kinase other than Rho kinase in the regulation of the myosin phosphatase activity and myofilament Ca2+-sensitivity in vascular smooth muscle
    K Hirano; K Nakamura; J Nishimura; H Kanaida  CIRCULATION  108-  (17)  100  -100  2003/10
  • Myofilament Ca2+ sensitivity in enhanced by cell penetrating peptide-mediated transduction of the myosin light chain phosphatase targeting subunit in intact porcine coronary artery
    K Hirano; DN Derkach; M Hirano; J Nishimura; H Kanaida  CIRCULATION  108-  (17)  101  -101  2003/10
  • HMG-CoA reductase inhibitor down-regulates expression of protease-activated receptor (PAR) 1 on the plasma membrane and reduces the responsiveness to thrombin in the human coronary artery smooth muscle cells
    T Yufu; K Hirano; D Bi; M Hirano; J Nishimura; H Kanaide  CIRCULATION  108-  (17)  202  -202  2003/10
  • Rho Kinase-Dependent and Independent Inhibition of Myosin Phosphatase and Ca^<2+>-Sensitization of the Contractile Apparatus in the Basilar Artery
    Hirano Katsuya; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  406  -406  2003/03
  • Functional Linkage of Reverse Mode Na^+Ca^<2+> Exchanger and Sarcoplasmic Reticulum Ca^<2+> Release in Porcine Coronary Arterial Smooth Muscle
    Yamanaka Jun; Nishimura Junji; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  407  -407  2003/03
  • An Important Role for the Na^+Ca^<2+> Exchanger in Decreasing Cytosolic Ca^<2+> Concentration by Isoprenaline in the Porcine Coronary Artery
    Yamanaka Jun; Nishimura Junji; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  407  -407  2003/03
  • A Novel Mechanism of Inactivation of Protease-Activated Receptor-1 by Thrombin in Endothelial Cells : An Additional Removal of the Tethered Ligand
    Hirano Katsuya; Nishumura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  526  -526  2003/03
  • Collagen Gel Constriction by Contractile Coronary Artery Smooth Muscle Cells : In-Vitro Model of Constrictive Remodeling
    Nishimura Junji; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  207  -207  2003/03
  • RhoA-Rho Kinase Pathway and Increased Ca^<2+> Sensitivity of the Vascular Smooth Muscle
    Nishimura Junji; Hirano Katsuya; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  67-  58  -58  2003/03
  • Inhibitors of HMG CoA reductase enhance the endothelial production of NO and attenuate vascular contractility in the isolated rabbit mesenteric artery.
    N Shiga; K Hirano; J Nishinura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  139P  -139P  2003
  • Theophylline decreases Ca2+ sensitivity of the contractile apparatus in the porcine tracheal smooth muscle.
    S Ise; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  250P  -250P  2003
  • Linkage of reverse mode Na+/Ca2+ exchanger and sarcoplasmic reticulum Ca2+ release in porcine coronary arterial smooth muscle.
    J Yamanaka; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  273P  -273P  2003
  • The contractile response to trypsin was enhanced by in vivo treatment with progesterone and estrogen in the isolated rat myometrium.
    M Aman; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  271P  -271P  2003
  • Mechanism of the contraction induced by N-formyl-L-methionyl-L-leucyl-L-phenylalanine in the guinea-pig taenia coli.
    H Kawata; K Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  122P  -122P  2003
  • Lipophilic inhibitors of HMG-CoA reductase suppress the response to thrombin in vascular smooth muscle.
    T Yufu; K Hirano; M Hirano; J Nishimura; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  158P  -158P  2003
  • The modulation of Ca2+ sensitivity regulates contractility of human detrusor smooth muscle.
    R Takahashi; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  263P  -263P  2003
  • Collagen gel constriction by contractile coronary artery smooth muscle cells: in-vitro model of constrictive remodeling.
    D Bi; J Nishimura; K Hirano; H Kanaide  JOURNAL OF PHARMACOLOGICAL SCIENCES  91-  127P  -127P  2003
  • A novel mode of inactivation of protease-activated receptor-1 by thrombin in endothelial cells: An additional proteolytic removal of the tethered ligand other than the N-terminal 41 residues
    K Hirano; J Nishimura; H Kanaide  CIRCULATION  106-  (19)  209  -210  2002/11
  • Temporal specific requirement of RhoA activity but not Rho-kinase for the G1 to S phase progression in the vascular endothelial cells
    K Hirano; M Hirano; J Nishimura; H Kanaide  CIRCULATION  106-  (19)  143  -143  2002/11
  • 平滑筋収縮装置のCa^<2+>感受性調節 : ミオシンリン酸化と脱リン酸化機構
    平野 勝也; DERKACH Dmitry N.; 平野 真弓; 西村 淳二; 金出 英夫  血管  25-  (2)  53  -61  2002/05
  • Transduction of the N-terminal fragment of MYPT1 enhances the contraction in intact smooth muscle of porcine coronary artery
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Circulation journal : official journal of the Japanese Circulation Society  66-  464  -464  2002/03
  • 平滑筋の緊張制御機序 ((社)日本麻酔科学会第49回大会講演特集号) -- (学術講演)
    金出 英夫; 平野 勝也; 西村 淳二  麻酔  51-  S33  -40  2002
  • Role of Na+/Ca2+ exchange in isoproterenol-induced relaxation of porcine coronary artery smooth muscle
    J Yamanaka; J Nishimura; K Hirano; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  69P  -69P  2002
  • HIV TAT protein-mediated transduction of the N-terminal fragments of MYPT1 enhanced contraction in intact smooth muscle of porcine coronary artery
    DN Derkach; K Hirano; M Hirano; J Nishimura; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  71P  -71P  2002
  • Rho-kinase potentiates the Ca2+ sensitivity with and without changing myosin phosphorylation in bovine middle cerebral artery
    Y Maeda; K Hirano; J Nishimura; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  254P  -254P  2002
  • Contraction of the cultured smooth muscle of porcine coronary artery
    D Bi; J Nishimura; K Hirano; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  253P  -253P  2002
  • Unproductive cleavage and desensitization of protease-activated receptor1 by trypsin in human enclotheial cells
    T Nakayama; K Hirano; J Nishimura; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  220P  -220P  2002
  • Mechanism for the theophylline-induced relaxation in porcine tracheal smooth muscle
    S Ise; J Nishimura; K Hirano; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  129P  -129P  2002
  • Role of PAR in the vascular system
    K Hirano; T Nakayama; DN Derkach; J Nishimura; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  54P  -54P  2002
  • The long-term deficiency of estrogen enhances the contractile response without affecting the Ca2+-sensitivity in arterial smooth muscle of the female rabbit
    M Koga; K Hirano; J Nishimura; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  71P  -71P  2002
  • The modulation of Ca2+sensitivity regulates the contractility of the rabbit corpus cavernosum
    R Takahashi; J Nishimura; K Hirano; H Kanaide  JAPANESE JOURNAL OF PHARMACOLOGY  88-  254P  -254P  2002
  • A guanylyl cyclase inhibitor, ODQ, is also a potent K+ channel blocker
    DN Derkach; K Hirano; J Nishimura; H Kanaide  CIRCULATION  104-  (17)  298  -298  2001/10
  • Cloning and characterization of a novel isoform of p27(kip1) in vascular endothelial cells
    K Hirano; M Hirano; Y Zeng; J Nishimura; H Kanaide  CIRCULATION  102-  (18)  226  -226  2000/10
  • The amino acid sequence of myosin regulatory light chain required for its subcellular localization and for the involvement in the Rho kinase-induced changes in cell shape
    T Yasutsune; J Nishimura; K Hirano; H Yasui; H Kanaide  CIRCULATION  102-  (18)  314  -314  2000/10
  • 32. 妊娠ラット子宮平滑筋におけるトロンビン受容体発現増加と収縮反応の増強
    新谷 可伸; 平野 勝也; 西村 淳二; 金出 英夫  Journal of smooth muscle research. Japanese section  4-  (1)  J  -32  2000/04
  • 41. モルモット結腸紐自発収縮に対するY-27632の効果
    家入 里志; 西村 淳二; 平野 勝也; 金出 英夫; 水田 祥代  Journal of smooth muscle research. Japanese section  4-  (1)  J  -37  2000/04
  • 0264 内皮由来過分極因子(EDHF)は内皮型NO合成酵素(eNOS)由来の非NO物質である可能性 : eNOSノックアウトマウスにおける検討
    的場 哲哉; 下川 宏明; 平川 洋二; 竹下 彰; 中島 幹夫; 平野 勝也; 金出 英夫  Japanese circulation journal  64-  251  -251  2000/03
  • IS162 Cloning of p27^, a Degradation-resistant Isoform of a Cyclin-dependent Kinase Inhibitor, in Vascular Endothelial Cells
    Hirano Katsuya; Hirano Mayumi; Nishimura Junji; Kanaide Hideo  Japanese circulation journal  64-  170  -170  2000/03
  • P749 トリプシンによる冠動脈の内皮依存性弛緩反応は内皮細胞Ca^<2+>貯蔵部位からのCa^<2+>放出によって制御されている
    平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  64-  647  -647  2000/03
  • P716 増殖型血管平滑筋細胞においてMAPキナーゼ系はアンギオテンシンII受容体発現を抑制する。
    平野 勝也; 平野 真弓; 西村 淳二; 金出 英夫  Japanese circulation journal  64-  639  -639  2000/03
  • P041 ミオシン軽鎖の特異的細胞内局在に必要なアミノ酸領域の決定とRhoキナーゼが引き起こす細胞形態変化への関わりについて
    安恒 亨; 西村 淳二; 平野 勝也; 金出 英夫; 安井 久喬  Japanese circulation journal  64-  469  -469  2000/03
  • 増殖型血管平滑筋細胞においてMAPキナーゼは電位依存性L型Ca2+ channelの発現を抑制する
    伊原 栄吉; 平野 勝也; 西村 淳二; 金出 英夫  Japanese Circulation Journal  64-  (Suppl.I)  254  -254  2000/03
  • Subcellular localization of caveolins in intact endothelial cells: Analysis with a fluorescent fusion protein expression system
    H Kanaide; T Yasutsune; J Nishimura; K Hirano; H Yasui  CIRCULATION  100-  (18)  416  -416  1999/11
  • Agonist-induced inhibition of myosin phosphatase via rho-rho kinase system is independent of Ca2+ in arterial smooth muscle
    YB Zhou; JJ Nishimura; K Hirano; H Kanaide  CIRCULATION  100-  (18)  417  -417  1999/11
  • 血管平滑筋の緊張制御に関する最新情報--Ca2+感受性調節におけるミオシン脱リン酸化酵素の役割 (10月第5特集 Vascular biology--分子生物学と病態・治療) -- (血管の構築)
    平野 勝也; 金出 英夫  医学のあゆみ  191-  (5)  332  -337  1999/10
  • ラット大動脈培養平滑筋細胞の増殖に伴う電位依存性及び容量性Ca2+流入機構の変化
    伊原 栄吉; 平野 勝也; 西村 淳二; 名和田 新; 金出 英夫  動脈硬化  26-  (8)  147  -147  1999/05
  • P-7. エストロゲン及びプロゲステロンによるモルモット結腸紐弛緩反応の相違
    家入 里志; 平野 勝也; 西村 淳二; 金出 英夫; 水田 祥代  Journal of smooth muscle research. Japanese section  3-  (1)  J  -52  1999/04
  • 0111 Vascular Endothelial Growth Factor (VEGF)による血管新生を、Ca^<2+>流入阻害剤SKF96365が阻止する
    川崎 純也; 平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  63-  (1)  175  -175  1999/03
  • 0585 平滑筋ミオシン脱リン酸化酵素調節サブユニットによる血管平滑筋収縮増強作用
    平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  63-  (1)  295  -295  1999/03
  • P316 caveolin-1およびcaveolin-2の細胞内局在 : 蛍光蛋白質との融合蛋白質発現系を用いた解析
    西村 淳二; 安恒 亨; 平野 勝也; 金出 英夫  Japanese circulation journal  63-  (1)  528  -528  1999/03
  • Differential role of four Ca2+ binding domains of calmodulin in smooth muscle contraction.
    N Niiro; J Nishimura; K Hirano; H Kanaide  CIRCULATION  98-  (17)  667  -667  1998/10
  • Ca2+ sensitivity of porcine renal artery is increased by the N-terminal mutants of 130 kDa subunit of smooth muscle myosin phosphatase.
    YB Zhou; K Hirano; J Nishimura; H Kanaide  CIRCULATION  98-  (17)  530  -531  1998/10
  • Troglitazone relaxes coronary artery by decreasing cytosolic calcium concentration
    J Kawasaki; K Hirano; Abe, I; H Kanaide; M Fujishima  JOURNAL OF HYPERTENSION  16-  S57  -S57  1998/06
  • Thapsigarginは豚腎動脈において内皮依存性の収縮と弛緩の両反応を引き起こす
    伊原 栄吉; 平野 勝也; 西村 淳二; 金出 英夫  Journal of smooth muscle research. Japanese section  2-  (1)  J  -44  1998/04
  • 0498 免疫抑制剤FK506は豚冠動脈平滑筋を弛緩させる
    安恒 亨; 平野 勝也; 西村 淳二; 金出 英夫; 安井 久喬  Japanese circulation journal  62-  229  -229  1998/02
  • P161 ブレフェルジンAは豚冠動脈において内皮由来過分極因子の産生を抑制する。
    平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  425  -425  1998/02
  • P591 血管平滑筋収縮におけるカルモジュリン機能の発現には第4Ca^<2+>結合部位が重要である。
    西村 淳二; 平野 勝也; 金出 英夫  Japanese circulation journal  62-  533  -533  1998/02
  • 0905 トロンビンが引き起こす内皮細胞Caシグナルと内皮依存性血管弛緩反応との相関
    水野 修; 平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  332  -332  1998/02
  • 0918 新規糖尿病薬トログリタゾンによるブタ冠動脈平滑筋細胞の弛緩機序
    川崎 純也; 平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  335  -335  1998/02
  • P692 ラット大動脈初代培養平滑筋細胞においてアンギオテンシンIIが引き起こす細胞質Ca濃度上昇反応の細胞密度依存性
    平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  559  -559  1998/02
  • P849 内皮細胞の細胞間接触は増殖停止とストレスファイバーの形態変化を誘導する。
    平野 真弓; 平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  598  -598  1998/02
  • P302 ラット大動脈初代培養平滑筋細胞におけるトロポミオシンα発現のスプライシング反応による調節
    平野 勝也; 西村 淳二; 金出 英夫  Japanese circulation journal  62-  460  -460  1998/02
  • SMOOTH-MUSCLE MYOSIN LIGHT-CHAIN PHOSPHATASE
    H SHIMIZU; M ITO; M MIYAHARA; K ICHIKAWA; S OKUBO; T KONISHI; M NAKA; T TANAKA; K HIRANO; DJ HARTSHORNE; T NAKANO  CIRCULATION  90-  (4)  356  -356  1994/10
  • MORPHOLOGICAL-CHANGES OF CULTURED-CELLS INDUCED BY PHOSPHATASE INHIBITORS
    K HIRANO; M HIRANO; R TAYLOR; N FUSETANI; H KARAKI; DJ HARTSHORNE  JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY  24-  S63  -S63  1992/05
  • GLIBENCLAMIDE-INDUCED RELAXATION OF RABBIT AORTIC SMOOTH-MUSCLE
    K YOSHITAKE; K HIRANO; H KANAIDE  CIRCULATION  82-  (4)  411  -411  1990/10
  • -1243-Ca^<2+>-FORCE RELATION IN SARAFOTOXIN INDUCED CONTRACTION OF RAT AORTIC STRIPS : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
    WATANABE Chiaki; HIRANO Katsuya; KANAIDE Hideo; NAKAMURA Motoomi  Japanese circulation journal  54-  (8)  1073  -1074  1990/08
  • -1092-EFFECTS OF DILTIAZEM ON, Ca^<2+>-TENSION RELATIONSHIP IN THE PORCINE CORONARY ARTERY : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
    HIRANO Katsuya; KANAIDE Hideo; NAKAMURA Motoomi  Japanese circulation journal  54-  (8)  1029  -1029  1990/08
  • -0555-EFFECTS OF THE ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITOR, CAPTOPRIL ON BRADYKININ-INDUCED CALCIUM TRANSIENT IN CULTURED BOCINE AORTIC ENDOTHELIAL CELLS
    Hirano Katsuya; Hirano Mayumi; Hisajima Hiroko; Kanaide Hideo; Nakamura Motoomi  Japanese circulation journal  54-  (7)  866  -866  1990/07
  • -113-ENDOTHELIN INDUCED Ca-INDEPENDENT CONTRACTION OF THE PORCINE CORONARY ARTERY : Cardiovascular Drugs : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
    Kodama Mayuko; Abe Shimako; Hirano Katsuya; Kai Hisashi; Kanaide Hideo; Nakamura Motoomi  Japanese circulation journal  53-  (6)  511  -511  1989/06
  • -194-CHARACTERISTICS OF INTRACELLULAR Ca-STORE SITES IN SMOOTH MUSCLE CELLS OF PORCINE CORONARY ARTERY : Coronary Circulation : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
    Hirano Katsuya; Abe Shimako; Kodama Mayuko; Kanaide Hideo; Nakamura Motoomi  Japanese circulation journal  53-  (6)  534  -534  1989/06
  • SEROTONIN-SENSITIVE INTRACELLULAR CALCIUM STORE IN VASCULAR SMOOTH-MUSCLE CELLS
    H KANAIDE; M HASEGAWA; H KAI; K HIRANO; M NAKAMURA  JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION  52-  (8)  734  -734  1988/08

Industrial Property Rights

  • 特許第6243844号:A therapeutic agent and a preventive agent for pulmonary hypertension comprising thrombin receptor antagonist as an active ingredient    2017/11/17
    HIRANO Katsuya  Eisai Co., Ltd.
  • 特許第3425620号:プロテアソーム分解に抵抗性を示す新規p27Kip1分子種の核酸及びアミノ酸配列と同蛋白質の発現ベクター及び発現細胞    2003/05/09
    平野勝也  九州大学長
  • トロンビン受容体アンタゴニストを有効成分とするくも膜下出血に伴う血管攣縮の治療剤
    特許出願PCT/JP2005/005068
  • Nucleic acid and amino acid sequences of the degradation-resistant novel isoform of p27Kip1, and expression plasmid and cells expressing the protein
    Patent3425620

Awards & Honors

  • 2024/03 Physiological Society of Japan Hiroshi and Aya Irisawa Memorial JPS Best Paper Award
     Critical role of Rho proteins in myosin light chain di-phosphorylation during early phase of endothelial barrier disruption 
    受賞者: Mayumi Hirano;Katsuya Hirano
  • 2023/03 Japanese Pharmacological Society Journal of Pharmacological Sciences Excellent Reviewer Award 2022
     
    受賞者: Katsuya Hirano
  • 2021/07 American Heart Association, Arteriosclerosis, Thrombosis and Vascular Biology Arteriosclerosis, Thrombosis and Vascular Biology Top Reviewer of 2020 Award
     
    受賞者: Katsuya Hirano
  • 2020/03 Japanese Pharmacological Society Journal of Pharmacological Sciences Excellent Reviewer Award 2019
     
    受賞者: Katsuya Hirano
  • 2017/03 Japanese Pharmacological Society Journal of Pharmacological Sciences Excellent Reviewer Award 2016
     
    受賞者: HIRANO Katsuya
  • 2014/08 Japan Society of Smooth Muscle Research Gold medal of logo mark design
     
    受賞者: Katsuya Hirano
  • 2013/03 The 29th Spasm Symposium 4th Best Paper Award
     JPN 
    受賞者: Yuichiro Kikkawa;Satoshi Matsuo;Katsuharu Kameda;Mayumi Hirano;Akira Nakamizo;Tomio Sasaki;Katsuya Hirano
  • 2011/07 The 27th Spasm Symposium, 2nd Best Paper Award
     Impaired feedback regulation of the receptor activity and the myofilament Ca2+ sensitivity contributes to increased vascular reactiveness after subarachnoid hemorrhage JPN 
    受賞者: Yuichiro Kikkawa;Katsuharu Kameda;Mayumi Hirano;Tomio Sasaki;Katsuya Hirano
  • 2010/04 The 26th Spasm Symposium, 1st Best Paper Award
     Enhanced contractile response of the basilar artery to platelet-derived growth factor in subarachnoid hemorrhage JPN 
    受賞者: Yoshihisa Maeda;Katsuya Hirano;Mayumi Hirano;Yuichiro Kikkawa;Katsuharu Kameda;Tomio Sasaki;Hideo Kanaide
  • 2010/03 財団法人宮田心臓病研究振興基金 平成２１年度未成年心臓血管病の教育研修に関する助成
     
    受賞者: 平野 勝也
  • 2002/02 The 31st Annual Meeting of Japanese Society of Circulation Research Young Investigator Award
     Vascular Smooth Muscle Contraction was Augmented by Introducing MYPT1, a Regulatory Subunit of Myosin Light Chain Phosphatase by Use of HIV TAT Protein JPN 
    受賞者: Katsuya Hirano

Research Grants & Projects

  • 血液凝固系による認知症発症機構の解明とその制御
    日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2024/06 -2027/03 
    Author : 平野 勝也
  • 血液凝固系の老化促進因子としての機能解明とその制御
    日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2024/04 -2027/03 
    Author : 平野 勝也; 倉原 琳; 橋本 剛; 山下 哲生
  • Elucidation and regulation of the senescence-accelerating function of coagulation system
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2022/04 -2027/03 
    Author : 平野 勝也
  • Development of strategies for improvement of insulin resistance of the aged by targeting the blood coagulation system
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2020/07 -2023/03 
    Author : 平野 勝也
     
    初年度の研究においてトロンビン受容体PAR1を欠損するマウスは、高脂肪食による肥満とインスリン抵抗性は野生型マウスと同定に生じるものの、加齢に伴うインスリン抵抗性が特異的に抑制されていることを見出した。これを受けて、PAR1拮抗薬を用いた薬理学介入が加齢に伴うインスリン抵抗性の発症に対して予防及び改善効果を発現するか否かを解析した。野生型マウスにおいて若齢期（8週齢）から80週齢まで長期間にわたりPAR1拮抗薬を飼料に混じて投与し、加齢に伴うインスリン抵抗性の発症予防効果を検討した。80週齢時のインスリン感受性および耐糖能を評価したところ、薬物投与群と薬物を投与しなかった対象群とに有意な差が認められなかった。さらに、高齢期（50週齢）から80週齢までPAR1拮抗薬を投与し、一旦成立したインスリン抵抗性および耐糖能障害を改善するかを検討した。80週齢時のインスリン抵抗性および耐糖能は、薬物投与群と薬物を投与しなかった対象群と差が認められなかった。 PAR1欠損マウスの脂肪組織の解析を行った。野生型マウスの脂肪組織（精巣上体周囲・腎臓周囲・肩甲骨間皮下・臀部皮下）は、加齢に伴い体重に占める湿重量比が増大するが、PAR1欠損マウスのそれは緩やかであった。 PAR1を全身性に欠損させることによる脂肪組織の減少と代謝改善効果（インスリン感受性や耐糖能）に関わる遺伝子発現変化を明らかに知るため、脂肪組織（腎臓周囲脂肪組織）・肝臓・腓腹筋のマイクロアレイ解析を行った。現在詳細を解析中である。
  • トロンビン受容体を標的とする新規肺高血圧治療法の開発
    Japan Society for the Promotion of Science：JSPS KAKENHI (No. 16K09518)
    Date (from‐to) : 2016/04 -2019/03 
    Author : HIRANO Katsuya
  • Elucidation of the molecular mechanism underlying the vascular dysfunction mediated by proteinase-activated receptor 1
    Japan Society for the Promotion of Science：JSPS KAKENHI (No. 15K09159)
    Date (from‐to) : 2015/04 -2018/03 
    Author : HIRANO Mayumi
     
    1. Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. 2. The Rho-Rho kinase pathway plays an important role in the peripheral localization of ppMLC and actin filament formation during the initial phase of the thrombin-induced endothelial barrier disruption. 3. The coagulation factor XIa induces Ca2+ signal via proteinase-activated receptor 1(PAR1) and L-type Ca2+channel in vascular smooth muscle cells. 4. Specific and increased expression of thrombin receptor PAR1 in pulmonary artery plays a key role in the pathogenesis of monocrotaline-induced pulmonary hypertension in rats.
  • Development of innovative and ultimate strategies for the treatment of cerebral vasospams with thrombin receptor as a therapeutic target
    Japan Society for the Promotion of Science：JSPS KAKENHI (No. 26670271)
    Date (from‐to) : 2014/04 -2016/03 
    Author : HIRANO Katsuya
     
    Cerebral vasospasm is one of critical complications that develop after subarachnoid hemorrhage and influence vital and neurologic prognosis of the patients. The present study aims to develop innovative and ultimate strategies for the treatment of cerebral vasospasm with thrombin receptor as a therapeutic target, based on the understanding of the central mechanism of pathogenesis of vasospasm. As a result, the present research elucidated the mechanisms underlying (1) impairment of desensitization of thrombin receptor PAR1, (2) thrombin-induced endothelial barrier disruption and (3) thrombin-induced store-operated calcium influx. All these aspects play a critical role for the development of cerebral vasospasm. The findings of the present study therefore consolidated the basis for the development of a novel therapeutic strategy with PAR1 as a therapeutic target for the prevention and treatment of cerebral vasospasm.
  • トロンビン受容体の活性制御異常の分子機構解明と新たな動脈硬化治療戦略の開発
    Japan Society for the Promotion of Science：JSPS KAKENHI (No. 24591118)
    Date (from‐to) : 2012/04 -2015/03 
    Author : HIRANO Mayumi
  • Elucidation of roles of proteinase-activated receptor in pulmonary hypertension and development of new therapeutic strategies
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2011/04 -2013/03 
    Author : HIRANO Katsuya; HIRANO Mayumi; ABE Kohtaro; ODA Yoshinao
     
    The present study yielded a new mechanism for endothelial barrier dysfunction. By using an animal model of pulmonary hypertension, a thrombin receptor antagonist was found to effectively prevent the development of pulmonary hypertension and prolonged the survival. The contractile responsiveness of pulmonary artery was found to be enhanced in pulmonary hypretension. As a result, thrombin receptor antagonist is suggested to be potentially useful as a new strategy for treatment of pulmonary hypertension. Proposals of Japanese and international patent for the invention of new treatment for pulmonary hypertension has been submitted.
  • Role of endothelial proteinase-activated receptors in the early phase of the development of vascular lesions.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2008 -2010 
    Author : Mayumi HIRANO; Katsuya HIRANO
     
    The present study elucidated the role of proteinase-activated receptors(PARs) in the early phase of the development of atherosclerotic vascular lesions. Thrombin increased endothelial permeability by two distinct pathways by activating PAR1 ; one is mediated by Gα13, and independent of Ca^<2+>, but dependent of Rho kinase-mediated MLC di-phosphorylation ; other is mediated by Gαq, and independent of MLC di-phosphorylation, but dependent on Ca^<2+>.
  • Role of proteinase-activated receptors in the dysregulation of vascular tone and the enhancement of the proliferative state in vascular lesions
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2005 -2006 
    Author : Katsuya HIRANO; 平野真弓
     
    Thrombus formation plays a critical role in the pathogenesis and pathophysiology of various vascular diseases. Proteinases, such as thrombin, activate proteinase-activated receptors (PARs), thereby exerting the vascular effects, including vasoconstriction, smooth muscle proliferation, vascular hyper-permeability, and production of the endothelium-derived vasoactive substances. However, the roles of PARs in the pathogenesis and pathophysiology of vascular diseases still remain to be elucidated. In the present study, we advanced our earlier studies on the physiological roles of PARs in the re...
  • Elucidation of the Intracellular Signaling Networks Involved in the Proliferation and Functional Differentiation Induced by Cell-cell Contact in Vascular Endothelial Cells.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2004 -2005 
    Author : Mayumi HIRANO; 平野勝也
     
    The present study demonstrated for the first time that the activation of Rac1 due to the formation of the cell-cell contact plays a critical role in the transcriptional up-regulation of p27^, thereby facilitating the contact-induced growth arrest in vascular endothelial cells. The induction of the homophilic cell-cell contact by adding the extra numbers of endothelial cells facilitated the cell cycle arrest, activated Rac1 but not RhoA, and up-regulated p27^ mRNA. Such up-regulation of p27^ mRNA was abolished by introducing the Rac1 inhibitor protein conjugated with the ce...
  • Elucidation of the mechanisms regulating the vascular tone and proliferation : Development of a novel technique to introduce protein into the intact cells and its applications
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2003 -2004 
    Author : Katsuya HIRANO
     
    By using a cell-penetrating peptide, which is found in the human immunodeficiency viral transcription factor Tat and is composed of 11 amino acids, I have developed a novel technique to rapidly, reversibly and quantitatively introduce proteins into the vascular tissues and cells. The cargo protein to be introduced into the cells was prepared as a recombinant protein fused to the cell-penetrating peptide using a bacterial expression system. I have developed the expression vectors. Using this new technique of the protein transduction, I have made the following contribution to the vascular bio...
  • 内皮・平滑筋・繊維芽細胞の三次元共培養による血管構築を摸した血管モデルの作製
    文部科学省：科学研究費補助金(萌芽研究)
    Date (from‐to) : 2002 -2003 
    Author : 金出英夫; 平野勝也; 西村淳二
     
    動脈硬化や血管形成術後の血管局所では、収縮性の亢進・攣縮を認めることがある。病変局所の平滑筋細胞や浸潤・増殖細胞において、収縮性亢進に関係する種々の酵素・蛋白質の発現変化が起こっている、と一般に考えられているが、その直接証拠は少ない。人工血管を作製し、これらの酵素・蛋白質の遺伝子を人工血管壁構成細胞に発現させることによって、収縮性の亢進・攣縮におけるその役割の直接証明が可能となる筈である。本年度は線維芽細胞のcell lineであるNIH3T3細胞を用いる事によって、等尺性収縮を測定可能な人工血管中膜モデルを作成した。さらにNIH3T3細胞に高効率に遺伝子導入できるベクターを開発し、CPI-17の過剰発現による線維芽細胞の収縮性の変化を検討した。CPI-17を過剰発現させた細胞とコラーゲンゲルを用いて作製した血管中膜モデルのリング標本は、PKCの活性化剤であるPDBuの投与により、10%FBSによる収縮の2-3倍の張力を発生した。一方、ベクターだけを発現させたコントロールではPDBuの投与により、むしろ基線以下に弛緩した。また、PKCによるリン酸化部位である38番目のThrをAlaあるはGluに変異させたCPI(CPI38AおよびCPI38E)では、PDBuによる収縮は得られず、コントロールと同様に弛緩した。CPI-17を過剰発現させた細胞のPDBuによる収縮は、PKC in...
  • Elucidation of the Intracellular Signal Networks Involved in the Growth Arrest induced by Cell-cell Contact in Vascular Endothelial Cells
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2002 -2003 
    Author : Mayumi HIRANO; 平野勝也
     
    1.The vascular endothelial cells ceased their growth upon formation of the tight cell-cell contact. The expression level of the cell cycle regulator p27^ was upregulated during the contact-induced growth arrest. This up-regulation was found to be due to transcriptional up -regulation.2.We have developed an assay system to determine the transcriptional regulatory element that responds to the formation of cell-cell contact in the cultured endothelia cells.3.We obtained a porcine genomic clone containing a full-length p27^ gene, and examined the promoter activity by using a 1500-nt...
  • Molecular mechanisms of vasospasm : Intracellular signaling network underlying the Ca^<2+> of smooth muscle cells.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2001 -2003 
    Author : Hideo KANAIDE; 平野勝也; 西村淳二
     
    Molecular mechanisms of vasospasm, especially the intracellular signaling network working for the Ca^<2+>-sensitization of smooth muscle cells, were determined. Following results were obtained. (1)Treatment of α-toxin-permeabilized rings with 1 mM ATPγS in the Ca^<2+>-free, ATP-free media enhanced the subsequent contraction induced by increment of Ca^<2+> concentration, and potentiated the Ca^<2+>-sensitivity, which was only partly inhibited by Rho-kinase inhibitor, Y-27632. We found a fundamental role of this new kinase other than Rho kinase in the regulation of the myosin phosphatase acti...
  • p27^新規分子種の同定とがん抑制因子としての機能解明
    文部科学省：科学研究費補助金(特定領域研究)
    Date (from‐to) : 2002 -2002 
    Author : 平野勝也
     
    細胞間接触により増殖を停止したブタ大動脈由来培養内皮細胞からサイクリン依存性キナーゼ阻害因子p27^の新たなアイソフォーム(p27^)をクローニングした。p27^のがん抑制因子としての機能を、細胞および個体レベルで明らかにすることを目的として、核移行、細胞周期制御及び分解抵抗性に関わる構造を明らかにした。また、自然高血圧発症ラット(SHR)と正常ラットの大動脈における2つのアイソフォームの発現解析から、組織増殖性とアイソフォーム発現の相関を、個体レベルで明らかにした。1.p27^は、領域153-168が有意な核移行に必要な配列であることが明らかになった。この領域には塩基性アミノ酸は一残基(K168)しか含まれない。不足する塩基性アミノ酸に代わり疎水性アミノ酸が機能を果たすという点で非典型的な2分裂型核移行シグナルであることを明らかにした。2.GFP発現系を用いて細胞増殖に及ぼす影響を解析し、p27^の増殖抑制作用を明らかにした。増殖抑制には、p27^と共通のN末端領域が必要である。3.6週齢SHRの大動脈にはp27^が、正常ラットではp27^が、主なアイソフォームとして発現する。週齢を重ねると、SHRにおいてもp27^発現が優位になる。正常ラット大動脈を培養...
  • The assessment of the contractility of the proliferating vascular smooth muscle
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2001 -2002 
    Author : Junji NISHIMURA; 平野勝也
     
    The purpose of the present study was to investigate the mechanism underlying the contractility of the proliferating airway smooth muscle. We could obtain the following results.1, Wd could construct the recombinant baculo virus vector, which can be infected with a high efficiency to the mammalian cell, by inserting the cytomegalo virus promoter and the cDNA of interest. This virus could successfully transfected to the NIH 3T3 fibroblast, porcine tracheal smooth muscle cells and porcine coronary smooth muscle cells. It took us long time to optimize the condition for the transfection. However,...
  • The assessment of the contractility of the proliferating airway smooth muscle.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2001 -2002 
    Author : Hidekazu SETOGUCHI; 平野勝也; 西村淳二
     
    The purpose of the present study was to investigate the mechanism underlying the contractility of the proliferating airway smooth muscle. We could obtain the following results.1. We could construct the recombinant baculo virus vector, which can be infected with a high efficiency to the mammalian cell, by inserting the cytomegalo virus promotor and the cDNA of interest. This virus could successfully transfected to the NIH 3T3 fibroblast, porcine tracheal smooth muscle cells and porcine coronary smooth muscle cells. It took us long time to opitimize the condition for the transfection. However...
  • Development of a system for the continuous and simultaneous measurement of vascular intracellular signalings and metabolism
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2001 -2002 
    Author : Hideo KANAIDE; 平野勝也; 西村淳二
     
    The only successful technique for "the continuous and simultaneous monitoring of the intracellular signalings, function and metabolism of blood vessels, without destructing the tissue structure" must be the front-surface fluorimetry. In the first year of this study, we have newly developed a system to carry out 3 sets of dual-wavelength front-surface fluorimetries simultaneously. Followings are characteristic features of the system: (1) In collaboration with the Japan Spectroscopic Co. (JASCO, Tokyo, Japan), we have developed a "shuttering system" which controls the on/off of the excitation...
  • Elucidation of the Mechanism for the cell cycle regulation by a novel isoform of p27^ in the vascular cells
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 2001 -2002 
    Author : Katsuya HIRANO
     
    A novel isoform of p27^, a cyclin-dependent kinase inhibitor, has been isolated from a cDNA library of porcine aortic endothelial cells. The N-terminal 162 amino acid of the new isoform was identical to those of p27^, while it contained a unique 18 amino acid C-terminus. The novel isoform was found to be resistant toward protease-mediated degradation, thus naming it p27^, a degradation resistant sioform of p27^. The region 153-168 was determined to be necessary for its significant nuclear localization. However, this region contains only one basic amino acid, and an ...
  • p27^新規分子種の同定とがん抑制因子としての機能解明
    文部科学省：科学研究費補助金(特定領域研究(C))
    Date (from‐to) : 2001 -2001 
    Author : 平野勝也
     
    正常細胞の増殖制御において最も重要で、生理的な役割を果たす細胞間接触による増殖停止に関して、そのメカニズムを明らかにするために、培養ブタ大動脈内皮細胞を用いてp27^の発現調節機構を明らかにした。内皮細胞を低密度(コンフルエント密度の25%)及び高密度(80%)の二つの異なる密度で植え付け、異なる時間経過で細胞同士の接触による増殖停止を誘導することにより、p27^蛋白質の発現変化と細胞周期進行との時間的相関を明らかにした。静止期の細胞を植え付け直すと、25%及び80%植え付け共に細胞は同調してS期に入り、20時間後にS期のピークに達した。その後、25%植え付けの場合は96時間後に、80%植え付けの場合は48時間後に細胞周期から外れた。核におけるp27^蛋白質の発現は、細胞周期に入る際に消失し、再び静止期に戻る際に回復した。このp27^蛋白質の発現変化は、Kip1 mRNAの発現変化を伴った。p27^蛋白質の分解活性は、細胞周期に入る際に増加したり、細胞周期から外れる際に低下することはなかった。Kip1 mRNAの安定性は増殖期に低下し、細胞周期から外れる時期及びコンフルエント(静止期)の時期に上昇した。Nuclear run-on assayによりKip1遺伝子の転写活性はKip1 mRNAの発現レベルと関連すること...
  • p27^新規分子種の同定とがん抑制因子としての機能解明
    文部科学省：科学研究費補助金(特定領域研究(C))
    Date (from‐to) : 2000 -2000 
    Author : 平野勝也
     
    血管内皮細胞cDNAライブラリーからサイクリン依存性キナーゼ阻害蛋白質p27^の新規分子種をクローニングした。新規分子種は、従来種とは異なるC末端を有するスプライスアイソフォームである。細胞抽出液を用いた分解アッセイを行ったことろ、従来種は増殖期の細胞抽出液によって1時間以内に分解されたのに対し、新規分子種は抵抗性であった。このことから新規分子種を、p27^(degradation-resistant isoform of p27^)と命名した。新規分子種を緑色蛍光色(GFP)との融合蛋白質として培養細胞に発現させると、従来種と同様に核へ局在することが明かとなった。GFP蛍光顕微鏡画像解析により核局在の程度を定量評価する方法を確立し、従来種の変異体を解析したところ、従来種の核移行シグナルは2分裂型であり、領域153〜166が核移行に必要最小限の領域であることを明らかにした。ところが、従来種と新規分子種の共通領域では核への特異的集積が消失した。このことから、新規分子種に特異的なC末端領域にも核移行に必要は配列があり、新規分子種の核移行シグナルも2分裂型であることが示唆された。GFP蛍光を指標に、フローサイトメーターを用いて、組換え蛋白質発現陽性及び陰性細胞の増殖を評価する方法を確立した。すなわち、遺伝子導入後各時点で、総細胞数を計測し、発現...
  • The role of the small GTP binding protein, rho, in the vascular smooth muscle.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 1999 -2000 
    Author : Junji NISHIMURA
     
    The small G protein, rho, has been shown to inhibit myosin light chain phosphatase (MLCP) through the phosphorylation of this enzyme by rho kinase. It was thus obvious that MLCP may be the key protein for the rho-mediated signal transduction to induce modulation of calcium sensitivity of the vascular smooth muscle. MLCP is composed of three subunits, namely, catalytic subunit, small regulatory subunit and large regulatory subunit. We focused on the function of two regulatory subunits of MLCP on the smooth muscle contractile apparatus in the present research program. The major findings were ...
  • Elucidation of the physiological role of myosin phosphatase in vascular endothelial cells and smooth muscle cells
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))
    Date (from‐to) : 1999 -2000 
    Author : 周応畢; Katsuya HIRANO; 平野勝也; 西村淳二
     
    The phosphorylation of myosin light chain plays a key role in the regulation of vascular tone. In addition, myosin phosphorylation is also essential in the regulation of motility and cytoskeletal organization in the non-muscle cells. The myosin phosphatase has been recently cloned and is composed of three subunits ; a 38 kDa catalytic subunit and two regulatory subunits of 20 kDa and 110 kDa. However, the physiological role of myosin phosphatase in the vascular smooth muscle and endothelial cells remained to be investigated.In this project, we first elucidated the regulatory role of the myo...
  • Development of the optical system for continuous and multi-factorial monitoring of the intracellular signaling network in endothelial and smooth muscle cells in vascular strips.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 1998 -2000 
    Author : Hideo KANAIDE; 平野勝也; 西村淳二
     
    The purpose of this study is to develop optical systems for continuous and multi-factorial monitoring of the intracellular signaling network in endothelial and smooth muscle cells in vascular strips. Using newly developed systems, we investigated the mechanisms underlying the regulation of the vascular tonus. Following results were obtained in this study : (1) We have developed a system to monitor changes in [Ca^<2+>]i and tension of the vascular strips simultaneously. This system was reported as a chapter "Measurement of [Ca^<2+>]i in smooth muscle strips using front-surface fluorimetry" i...
  • 血管内皮細胞カドヘリン複合体結合蛋白質の同定と生理機能の解明
    文部科学省：科学研究費補助金(奨励研究(A))
    Date (from‐to) : 1998 -1999 
    Author : 平野勝也
     
    細胞間接触による血管内皮細胞増殖の抑制は、内皮細胞を機能的成熟に導く重要な機構である。本研究では、(1)細胞間接触の受容から核における増殖制御に至る細胞質及び核内シグナル伝達系(Ca情報、蛋白質リン酸化あるいは脱リン酸化酵素、転写因子、細胞周期制御蚤白質など)を分子細胞生物学的に解明すること、(2)細胞接着因子の一つ内皮型カドヘリンとその機構複合体の形成に関与する構造を、ツーハイブリッド法を用いて明らかにすることを目的として研究を行った。1.培養内皮細胞の増殖及び細胞周期を、Bromod eoxyuridine(BrdU)の取り込みで評価する方法を確立した。コンフルエントの細胞は、BrdU陽性細胞の割合は0.1%であり、細胞間接触の完成した時期の内皮細胞は増殖を停止していることが明かとなった(Hirano et al.,1999)。2.静止期にある培養内皮細胞及び生体内皮細胞(大動脈弁条片)における、Ca情報伝達機構を明らかにし、血管緊張調節における内皮細胞内Ca情報伝達の役割を明らかにした(Ihara et al.,1999;Kawasaki et al.,1999)3.内皮細胞cDNAライブラリーからミオシン脱リン酸化酵素調節サブユニット(MYPT1)をクローニングした (Hirano et al.,1999)。初めに平滑筋で同定されたMYPT1と相同性が高く、また、細胞...
  • 血管平滑筋トロポミオシンの発現調節に関与するスプライシング因子の同定
    文部科学省：科学研究費補助金(特定領域研究(A))
    Date (from‐to) : 1998 -1998 
    Author : 平野勝也
     
    血管平滑筋の形質転換に伴うαトロポミオシン・アイソフォーム変換の調節に関与する情報伝達機構を明らかにする事を目的として今年度の研究を行った。1. 平滑筋細胞を培養すると、培養3〜5日目を最大に、一過性に非平滑筋型αトロポミオシンの発現が増加した。非平滑筋型アイソフォームの発現増加に一致して、BrdUの取り込みが、培養3日目を最大に一過性に増加した。2. コンフルエントの細胞を、2日間血清を含まない培地で培養し、再び血清刺激を行うと、刺激1.5時間で、非平滑筋型αトロポミオシンの発現が増加した。血清濃度1%から増強作用が認められ、3%で最大効果に達した。この時、非平滑筋型アイソフォームの発現は刺激前の約2倍に増加した。3. 血清再添加による非平滑筋型アイソフォーム発現増加は、H-7処理により完全に抑制されたが、wortmanninは有意な抑制作用を示さなかった。4. phorbil 12,13 dibutyrateは、血清刺激と同様に、刺激1.5時間で非平滑筋型アイソフォーム発現を増加させた。1nMから増強作用が認められ、100nMで最大効果に達した。この時、非平滑筋型アイソフォームの発現は刺激前の約2倍に増加した。5. Phorb01 12,13 dibutyrateは、1.5時間あるいは24時間刺激を行っても、BrdU取り込みには影響を与えなかった。血清刺激も、1.5時間で...
  • Studies on molecular cell biology of the inhibition of coronary vasoconstriction and intimal thickening.
    Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(一般研究(A), 基盤研究(A))
    Date (from‐to) : 1995 -1998 
    Author : Hideo KANAIDE; 小林誠; 西村淳二; 平野勝也; 小林誠
     
    For the prevention of coronary vasoconstriction and intimal thickening, following studies were performed : In smooth muscle and endothelial cells, (1)the characterization of the intracellular signal transduction networks, (2) the elucidation of the relationships between cell cycle progression and the cellular functions, and (3) the elucidation of the relationships between signaling factors and cellular functions/structures. (4) The clinical applicatopn of the results of these experimental studies.Following results/conclusions were obtained : (1) We have developed a method to monitor [Ca]i c...
  • 血管平滑筋細胞の分化に関与する平滑筋特異的スプライシング因子の同定
    文部科学省：科学研究費補助金(重点領域研究)
    Date (from‐to) : 1997 -1997 
    Author : 平野勝也
     
    本研究は、平滑筋特異的スプライシング因子を同定するために、培養平滑筋細胞を用いて、平滑筋細胞の形質変換に伴うトロポミオシンのアイソフォ-ムの変換調節機構を明らかにすることを目的として行った。培養細胞には、ラット大動脈平滑筋細胞の初代培養を用いた。10%胎児牛血清を含む培地で培養し、コンフルエントに達した細胞を無血清培地で2日間培養して、実験に用いた。α-トロポミオシンアイソフォ-ム発現の解析は、逆転写-PCRにより行った。ラットα-トロポミオシンのエクソン1、2、3、4に特異的なプライマ-を作製し、平滑筋細胞から精製した総RNAを1μg用い、RT-PCRによりトロポミオシン発現を解析した。1.無血清培地で培養した平滑筋細胞を、再び血清で刺激すると1.5時間以内に、エクソン3を含む非平滑筋型アイソフォ-ムの発現が増加した。エクソン1、エクソン2の発現は変化しなかった。血清濃度1%から増強作用が認められ、3%で最大効果に達し、エクソン3の発現は刺激前の約2倍になった。2.血清によるエクソン3の発現増強は、1-10μM H-7の前処理により完全に抑制された。wortmanninは、0.1μMの濃度において、不完全な抑制作用を示した。3.フォルボ-ル12,13ジブチレ-トで平滑筋を刺激すると、エクソン3の発現が増加した。1nMから増強作用が認められ、100nMで最大効果に達し、エクソ...

Committee Membership

  • 2023/03 - Today   Physiological Society of Japan   Editorial board of the Journal of Physiological Sciences
  • 2022/06 - Today   Physiological Society of Japan   Member of the Certification Committee of Physiology Educator
  • 2020/03 - Today   Physiological Society of Japan   Vice President
  • 2019/11 - Today   Physiological Society of Japan   member of the committee for meetings
  • 2016/08 - Today   Japan Society of Smooth Muscle Research   Board member
  • 2016/08 - Today   Japan Society of Smooth Muscle Research   Member of the committee for Academic Program
  • 2016/08 - Today   Japan Society of Smooth Muscle Research   Member of the committee for Publications
  • 2016/08 - Today   Japan Society of Smooth Muscle Research   Member of the committee for Future planning
  • 2015/02 - Today   Japanese Society of Circulation Research   Board member
  • 2014/11 - Today   Frontiers in Cardiovascular Medicine   Frontiers in Cardiovascular Medicine, Review editor, Review editor
  • 2014/06 - Today   Japanese Pharmacological Society   Editorial adviser of Journal of Pharmacological Sciences
  • 2013/07 - Today   International Society of Heart Research, Japan Section   Council member
  • 2013 - Today   Japan Society of Smooth Muscle Research   Council member
  • 2012/04 - Today   Physiological Society of Japan   Member of the committee for Publications and Public Relations
  • 2010/11 - Today   Frontiers in Integrative and Regenerative Pharmacology   Frontiers in Integrative and Regenerative Pharmacology, Review editor
  • 2022/07 -2023/06   Physiological Society of Japan   Awards committee, chairperson
  • 2018/03 -2023/06   Physiological Society of Japan   Member of the committee for Awards
  • 2020/08 -2021/08   Japan Society of Smooth Muscle Research   The president of the 63rd Annual Meeting of Japan Society of Smooth Muscle Research
  • 2016/11 -2018/03   Physiological Society of Japan   95th Annual Meeting of the Physiological Society of Japan
  • 1998/12 -1999/12   International Society of Heart Research, Japan Section   Secretary general, the 16th Annual Meeting of Japanese section of International Society of Heart Research

Social Contribution

  • Journal of Smooth Muscle Research
    Date (from-to) : 2022/08-Today
    Role : Chief
    Category : Pr
    Sponser, Organizer, Publisher  : Japan Society of Smooth Muscle Research
    Event, Program, Title : Journal of Smooth Muscle Research

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