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Narumi Takako

  • Faculty of Agriculture
  • Department of Applied Biological Science
  • Associate Professor
Last Updated :2025/04/24

Researcher Information

Degree

  • Master ( Agriculture Science )（Tohoku University）

J-Global ID

Research Interests

  • 花き園芸学   floricultural science   

Research Areas

  • Environmental science/Agricultural science / Horticulture
  • Environmental science/Agricultural science / Plant genetics and breeding
  • Life sciences / Morphology, anatomy
  • Life sciences / Plants: molecular biology and physiology

Academic & Professional Experience

  • 2008 - 2012  国立大学法人香川大学農学部, 助教
  • 2012  - 国立大学法人香川大学農学部, 准教授
  • 2005 - 2008  独立行政法人農業・生物系特定産業技術研究機構花き研究所, 特別研究員
  • 2005  独立行政法人農業・生物系特定産業技術研究機構果樹研究所, 非常勤研究員
  • 2002  - Grant-in-Aid from Japan Society for the Promotion
  • of Science (No.14360013)

Education

  •        - 2005  Tohoku University  農学研究科  環境修復生物工学
  •        - 2002  Tohoku University  Graduate School, Division of Agriculture  Bioremediation and Biotechnology
  •        - 2002  Tohoku University  農学研究科  環境修復生物工学
  •        - 2000  Aomori University  Faculty of Engineering  Bioengineering
  •        - 2000  Aomori University  Faculty of Engineering  Department of Bioscience and Biotechnology

Association Memberships

  • 日本植物生理学会   園芸学会   International Society for Horticultural Science: ISHS   日本植物バイオテクノロジー学会   

Published Papers

Books etc

  • The Science of Horticulture, vol. 1・Transgenic ornamentals with reduced ethylene production and perception
    2011

Conference Activities & Talks

  • 効率的な形質転換キク作出技術の開発  [Not invited]
    園芸学会  2011
  • シロイヌナズナAP1キメラリプレッサー遺伝子を導入したキクの花成  [Not invited]
    園芸学会平成22年度春季大会  2010
  • FT形質転換ギクにおける導入FT遺伝子発現量の経時的変化と花芽分化の関係  [Not invited]
    園芸学会平成22年度春季大会  2010
  • シロイヌナズナの発生・分化関連転写因子を用いた花きの効率的な形質改変手法の確立  [Not invited]
    第51回日本植物生理学会年会  2010
  • 数種花きの花弁表皮細胞形態の品種間差  [Not invited]
    園芸学会平成２２年度中四国支部大会  2010
  • Morphological change in the transgenic torenia plants expressing Arabidopsis TCP3 chimeric repressor  [Not invited]
    28th International Horticultural Congress  2010
  • Transgenic chrysanthemum expressing Arabidopsis chimeric AP1 repressor show in vitro flowering under long day conditions  [Not invited]
    28th International Horticultural Congress  2010
  • Floral transition in FT transformed chrysanthemum occurs in the shoot maintaining expression of introduced FT gene  [Not invited]
    28th International Horticultural Congress  2010
  • シロイヌナズナ由来TCP3SRDX遺伝子導入がトレニアの花弁形態形成関連遺伝子発現に及ぼす影響  [Not invited]
    園芸学会平成22年度秋季大会  2010
  • キクタニギクから単離したFT/Hd3a相同遺伝子CsFTL3の機能解析  [Not invited]
    園芸学会平成22年度秋季大会  2010
  • キク花成の暗期中断反応におけるフィトクロームの関与および分光感度  [Not invited]
    園芸学会平成22年度秋季大会  2010
  • シロイヌナズナFT遺伝子を導入したキクの軸上の花成の勾配とFT遺伝子発現  [Not invited]
    園芸学会平成21年度春季大会  2009
  • Transgenic torenia expressing chimeric AGAMOUS repressor exhibits serrated petals as those induced by cytokinin application  [Not invited]
    23rd International Eucarpia symposium  2009
  • Transgenic Torenia with Novel Floral Traits by Collective Introduction of Chimeric Repressors for Arabidopsis Transcription Factors  [Not invited]
    23rd International Eucarpia symposium  2009
  • Redesigning Floral Architecture: Efficient Modification of Agronomic Traits by CRES-T  [Not invited]
    23rd International Eucarpia symposium  2009
  • シロイヌナズナ由来のTCP3キメラリプレッサー遺伝子を導入したトレニアの形態について  [Not invited]
    園芸学会平成21年度秋季大会  2009
  • 校正機能不活性化型DNAポリメラーゼδを用いた変異技術によるトレニアの形質改変  [Not invited]
    園芸学会平成21年度秋季大会  2009
  • EIN3キメラリプレッサー遺伝子を導入したエチレン非感受性形質転換キクの特性  [Not invited]
    園芸学会平成20年度秋季大会
  • シロイヌナズナ由来EIN3キメラリプレッサー遺伝子導入によるエチレン非感受性キクの作出  [Not invited]
    園芸学会平成19年度秋季大会

Works

  • エチレン非感受性形質転換キクの作出
    2002 -2005
  • Generation and characterization of transgenic Chrysanthemum insensitive to ethylene by Agrobactrium-mediated gene transfer
    2002 -2005

MISC

Research Grants & Projects

  • 枝変わりカーネーション品種群を用いた枝変わり花色変異機構の解明
    日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2019/04 -2020/03 
    Author : 鳴海 貴子; 高村 武二郎
     
    本年度は, 「ミニティアラシリーズ」の 各花弁発達段階における花色素分析およびフラボノイド生合成関連遺伝子の解析を行った. 花色素分析の結果, 主要花色素として未同定のアントシアニン（An1）, ペラルゴニジン3,5ジグルコシド（Pg3,5dG）およびカルコン2’グルコシド（Ch2’G）が検出され. それらの花色素の蓄積量は, 各品種で異なることが明らかとなった. フラボノイド生合成関連遺伝子の解析の結果, カルコン異性化酵素遺伝子（CHI1およびCHI2）, ジヒドロフラボノール4-還元酵素遺伝子（DFR）, グルタチオンS-転移酵素遺伝子（GSTF2）およびCh2’Gの蓄積に関与する遺伝子であるカルコン2’ 配糖化酵素遺伝子（CHGT3）は, 各品種で発現パターンが異なることが明らかとなった. ‘ミニティアラベビーピンク’は‘ミニティアラピンク’と比べてCHI1, CHI2およびDFRの発現量が少なく, さらにGSTF2がほとんど発現していなかった. また‘ミニティアラピンク’と比べてAn1およびPg3,5dGの花弁への蓄積量が少なかった. 以上の結果から, ‘ミニティアラピンク’から‘ミニティアラベビーピンク’への花色変異は, これらの遺伝子の低発現により, これらの花色素の蓄積量が減少したことに起因すると推定された. ‘ミニティアラミルクホワイト’は‘ミニティアラピンク’と比べてCHI1およびCHI2の発現量が少なく, さらにDFRおよびGSTF2がほとんど発現していなかった. また‘ミニティアラミルクホワイト’はAn1およびPg3,5dGを花弁にほとんど蓄積していなかった. 以上の結果から, ‘ミニティアラピンク’から‘ミニティアラミルクホワイト’への花色変異は, これらの遺伝子の低発現により, これらの花色素の蓄積量が減少したことに起因すると推定された.
  • Elucidation of the shape control mechanism of petal epidermal cells determining the tone and texture of petal
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2013/04 -2018/03 
    Author : Narumi-Kawasaki Takako
     
    Flower color is determined by the components of pigment and the shapes of the petal epidermal cells. The shape of petal epidermal cells influences the tone and texture of petals. Three types of petal epidermal cell-related transcription factor genes (TfMYBML1～3) were isolated from Torenia fournieri L., which were homologous with genes reported in Antirrhinum majus L. in a previous study. The localization of gene expression and gene function by production of transgenic torenias of these genes was analyzed. The results indicated that TfMYBML1 is a transcription factor which does not affect the shape of petal epidermal cell, and TfMYBML2 and TfMYBML3 are transcription factors which partially affect the shape of the epidermal cell of the petal lips. Most importantly, TfMYBML3 was found to mainly regulate the shape of epidermal cell of the distinctive yellow blotch existing at the abaxial petal of torenia. TfMYBML1 and TfMYBML2 can be ruled out as main factors.
  • Mechanism of temperature-dependent petal coloration in chrysanthemum
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2014/04 -2017/03 
    Author : Fukai Seiichi; DOUSONO Mituru; SASAKI Katsutomo; Theeraniti Puangkrit
     
    Accumulation of anthocyanin was remarkably decreased at 30℃ compared to 20 ℃. When inflorescences were exposed to high and cool temperature by developmental stage of inflorescence the accumulation of anthocyanins occurred most intensely during the period from petal appearance to petal elongation stage. This period was most sensitive to temperature. Anthocyanin biosynthesis related genes are determined. A CmplCHI, and CmplANS, and two CmplCHS, CmplF3H and CmplDFR were isolated and showed gene sequences similar to chrysanthemum known sequences. All genes were contained the specific conserved region as known anthocyanin biosynthesis gene. Expression of all genes was depressed at 30°C. 50 ppm and 100 ppm BA treatment increased petal anthocyanin accumulation. This treatment was shown to be highly effective in the stage where accumulation of anthocyanins is most active among the developmental stages of inflorescence.
  • マーガレットの開花特性に関する研究
    Date (from‐to) : 2013
  • Study on flowering characteristics in Marguerite
    Date (from‐to) : 2013
  • Study on flower color expression mechanism in sugar loaded flowers
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2010 -2012 
    Author : FUKAI Seiichi; TAKAMURA Takejirou; NARUMI Takako
     
    It is well known that the sugar addition to water improves the flower color in cut flowers. In this study, flower color change by sugar addition, the composition and quantitativealteration of pigments, and change of gene expression of anthocyanin-biosynthesis related genes were studied, and development of the practical sugar addition method was aimed. In cut carnation flowers, cut flowers stored with higher concentration of sucrose bloomed earlier with larger flowers. Sugar accumulation was observed and the peak of ethylene production was delayed when the cut flowers were stored with 5% sucrose solution for 3 weeks. Cut flowers stored for four to eight weeks with sucrose 5% showed about three weeksvase life. Two main anthocyanins were accumulated during the storage with sucrose. Although transcription factor gene DcAN2 assumed to be a sugar-induced type was obtained from the carnation petal, it did not react to exogenous sucrose. The expression level of CHS, CHI, DFR, and ANS increased during storage with sucrose solution. The expression level of CHS, DFR, ANS, and DcAN2 also increased during longevity test. In cut gloriosaflowers. Although no difference in flower color was observed in the 1st and 2 floretsof cut flowers with or without sucrose treatment, the upper florets in the cut flowers kept in sucrose solution showed lower L values and higher C values. Moreover, poor flower color expression in the florets in middle position was observed. The anthocyanin content of florets at higher positions decreased rapidly in the cut flowers kept in water. When the cut flowers were kept in sucrose solution the anthocyanin content of 4thand 5thflorets recovered to equivalent anthocyanin content level of the first floret. Although sugar content of florets at higher positions decreased in cut flowers kept in water, those at higher positions increased in cut flowers kept in sucrose solution. The results showed that the poor color expression was not due to shortage of sugar in florets. Gene expression of GsDFR and GsANS was high at the 3rd to 5 flowers, suggesting that the poor color expression related to the expression of downstream genes in anthocyanin biosynthesis.
  • Development of technology for creating novel flower color patterns via shape control of petal epidermal cells
    Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2009 -2012 
    Author : NARUMI Takako
     
    In this study, TfMYBML1, TfMYBML2, and TfMYBML3 isolated from Torenia fournieri were found to inhibit the expression patterns of different genes according to petal development of torenia. Analysis of TfMYBML1-, TfMYBML2-, and TfMYBML3-suppressed transgenic torenia plants suggested that TfMYBML3 is closely related to the epidermal cell differentiation of a yellow blotch on the lower petal. However, each transgenic torenia plant constitutively overexpressing TfMYBML1, TfMYBML2, and TfMYBML3 showed the same phenotype as the wild-type plant. These results suggest that TfMYBML1, TfMYBML2, and TfMYBML3 are not the main factors responsible for controlling epidermal cell shape in the petals of T. fournieri
  • キクの花成メカニズムの解明
    Date (from‐to) : 2009
  • The molecular mechanisms of flowering in chrysanthemum
    Date (from‐to) : 2009
  • 花弁表皮細胞の形態制御機構の解明
    Date (from‐to) : 2008
  • Elucidation of shape regulatory mechanism of petal epidermal cells
    Date (from‐to) : 2008
  • CRES-T法による新規形質花きの作出と分子育種技法としての確立
    その他の研究制度
    Date (from‐to) : 2005 -2007
  • エチレン非感受性形質転換キクの作出
    ２１世紀グリーンフロンティア研究
    Date (from‐to) : 2002 -2005
  • Generation and characterization of transgenic Chrysanthemum insensitive to ethylene by Agrobactrium-mediated gene transfer
    0105 (Japanese Only)
    Date (from‐to) : 2002 -2005
  • キクのエチレンレセプター遺伝子の単離と発現解析
    Date (from‐to) : 2000 -2004
  • cDNA cloning of a chrysanthemum ethylene receptor and analysis of gene expression
    Date (from‐to) : 2000 -2004

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